RTN2 Recombinant antibody

• 

  WB analysis using 86162-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86162-1-RR (RTN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

RTN2 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86162-1-RR

Clone No.250823C1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Neuroendocrine-specific protein-like 1, Neuroendocrine-specific protein-like I, NSPL1, NSPLI, NSP-like protein 1

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue, mouse skeletal muscle tissue, rat skeletal muscle

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86162-1-RR targets RTN2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RTN2 fusion protein Ag1655
• Full Name: reticulon 2
• Calculated Molecular Weight: 59 kDa, 51 kDa, 22 kDa
• Observed Molecular Weight: 20-23 kDa
• GenBank Accession Number: BC014244
• Gene Symbol: RTN2
• Gene ID (NCBI): 6253
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75298
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. Human RTN2 gene produces three isoforms termed RTN2-A, RTN2-B, and RTN2-C. Isoform RTN2-C has a calculated molecular weight of 22 kDa and is highly expressed in skeletal muscle.