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  • KD/KO Validated

RUVBL2 Polyclonal antibody

RUVBL2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 10195-1-AP


48 kDa TBP interacting protein, CGI 46, ECP 51, ECP51, INO80 complex subunit J, INO80J, Repressing pontin 52, REPTIN, Reptin 52, RuvB like 2, RuvB like 2 (E. coli), RUVBL2, RVB2, TAP54 beta, TIH2, TIP48, TIP49B

Tested Applications

Positive WB detected inC2C12 cells, Daudi cells, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells, mouse brain tissue
Positive IHC detected inhuman testis tissue, human kidney tissue, human placenta tissue, human skin tissue, human brain tissue, human heart tissue, human lymphoma tissue, human gliomas tissue, human spleen tissue, human lung tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10195-1-AP targets RUVBL2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RUVBL2 fusion protein Ag0253
Full Name RuvB-like 2 (E. coli)
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC000428
Gene Symbol RUVBL2
Gene ID (NCBI) 10856
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RuvB-like 2 (E. coli) (RUVBL2, synonyms: ECP51, TIP48, CGI-46, TIP49B) is the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that RUVBL2 has both ATPase and DNA helicase activities.


Product Specific Protocols
WB protocol for RUVBL2 antibody 10195-1-APDownload protocol
IHC protocol for RUVBL2 antibody 10195-1-APDownload protocol
IF protocol for RUVBL2 antibody 10195-1-APDownload protocol
IP protocol for RUVBL2 antibody 10195-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mol Cell

Metabolic stress controls mTORC1 lysosomal localization and dimerization by regulating the TTT-RUVBL1/2 complex.

Authors - Sang Gyun Kim

Sci Adv

Synthetic lethality by targeting the RUVBL1/2-TTT complex in mTORC1-hyperactive cancer cells.

Authors - Seung Ho Shin
  • KD Validated

Nat Commun

Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.

Authors - Jiawei Zhang

J Clin Invest

circular RNA cia-MAF drives self-renewal and metastasis of liver tumor-initiating cells via transcription factor MAFF.

Authors - Zhenzhen Chen

Sci Adv

ETV7 is an essential component of a rapamycin-insensitive mTOR complex in cancer.

Authors - Franklin C Harwood


LncRNA HAND2-AS1 promotes liver cancer stem cell self-renewal via BMP signaling.

Authors - Yanying Wang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Deyong (Verified Customer) (03-11-2022)

It worked well in H1 human embryonic stem cells.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Human embryonic stem cell