|Positive WB detected in||C2C12 cells|
|Positive IP detected in||HeLa cells, mouse brain tissue|
|Positive IHC detected in||human testis tissue, human kidney tissue, human placenta tissue, human skin tissue, human brain tissue, human heart tissue, human lymphoma tissue, human gliomas tissue, human spleen tissue, human lung tissue, human ovary tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10195-1-AP targets RUVBL2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RUVBL2 fusion protein Ag0253|
|Full Name||RuvB-like 2 (E. coli)|
|Calculated molecular weight||51 kDa|
|Observed molecular weight||51 kDa|
|GenBank accession number||BC000428|
|Gene ID (NCBI)||10856|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RuvB-like 2 (E. coli) (RUVBL2, synonyms: ECP51, TIP48, CGI-46, TIP49B) is the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that RUVBL2 has both ATPase and DNA helicase activities.
|Product Specific Protocols|
|WB protocol for RUVBL2 antibody 10195-1-AP||Download protocol|
|IHC protocol for RUVBL2 antibody 10195-1-AP||Download protocol|
|IF protocol for RUVBL2 antibody 10195-1-AP||Download protocol|
|IP protocol for RUVBL2 antibody 10195-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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