|Positive WB detected in||C2C12 cells|
|Positive IP detected in||HeLa cells, mouse brain tissue|
|Positive IHC detected in||human testis tissue, human skin tissue, human lung tissue, human heart tissue, human kidney tissue, human brain tissue, human placenta tissue, human spleen tissue, human ovary tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10195-1-AP targets RUVBL2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RUVBL2 fusion protein Ag0253|
|Full Name||RuvB-like 2 (E. coli)|
|Calculated molecular weight||51 kDa|
|Observed molecular weight||51 kDa|
|GenBank accession number||BC000428|
|Gene ID (NCBI)||10856|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RuvB-like 2 (E. coli) (RUVBL2, synonyms: ECP51, TIP48, CGI-46, TIP49B) is the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that RUVBL2 has both ATPase and DNA helicase activities.
|Product Specific Protocols|
|WB protocol for RUVBL2 antibody 10195-1-AP||Download protocol|
|IHC protocol for RUVBL2 antibody 10195-1-AP||Download protocol|
|IF protocol for RUVBL2 antibody 10195-1-AP||Download protocol|
|IP protocol for RUVBL2 antibody 10195-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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