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  • KD/KO Validated

RUVBL2 Polyclonal antibody

RUVBL2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10195-1-AP

Synonyms

48 kDa TBP interacting protein, CGI 46, ECP 51, ECP51, INO80 complex subunit J, INO80J, Repressing pontin 52, REPTIN, Reptin 52, RuvB like 2, RuvB like 2 (E. coli), RUVBL2, RVB2, TAP54 beta, TIH2, TIP48, TIP49B



Tested Applications

Positive WB detected inC2C12 cells
Positive IP detected inHeLa cells, mouse brain tissue
Positive IHC detected inhuman testis tissue, human kidney tissue, human placenta tissue, human skin tissue, human brain tissue, human heart tissue, human lymphoma tissue, human gliomas tissue, human spleen tissue, human lung tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10195-1-AP targets RUVBL2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RUVBL2 fusion protein Ag0253
Full Name RuvB-like 2 (E. coli)
Calculated molecular weight 51 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC000428
Gene symbol RUVBL2
Gene ID (NCBI) 10856
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RuvB-like 2 (E. coli) (RUVBL2, synonyms: ECP51, TIP48, CGI-46, TIP49B) is the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that RUVBL2 has both ATPase and DNA helicase activities.

Protocols

Product Specific Protocols
WB protocol for RUVBL2 antibody 10195-1-APDownload protocol
IHC protocol for RUVBL2 antibody 10195-1-APDownload protocol
IF protocol for RUVBL2 antibody 10195-1-APDownload protocol
IP protocol for RUVBL2 antibody 10195-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Biochem Biophys Res Commun

Identification and characterization of R2TP in the development of oral squamous cell carcinoma.

Authors - Tetsuo Kiguchi
  • KD Validated
humanWB

Biochem Biophys Res Commun

HEATR1, a novel interactor of Pontin/Reptin, stabilizes Pontin/Reptin and promotes cell proliferation of oral squamous cell carcinoma.

Authors - Akihiko Nakamura
humanWB

Onco Targets Ther

WHSC1 Promotes Cell Proliferation, Migration, and Invasion in Hepatocellular Carcinoma by Activating mTORC1 Signaling.

Authors - Jingjing Dai
humanIHC

Cancer Cell Int

Multilevel regulation of RUVBL2 expression predicts poor prognosis in hepatocellular carcinoma.

Authors - Tao Yan
humanWB

J Biol Chem

RNA-binding proteins and heat-shock protein 90 are constituents of the cytoplasmic capping enzyme interactome.

Authors - Jackson B Trotman
mouse

RNA

Mechanism of the AAA+ ATPases pontin and reptin in the biogenesis of H/ACA RNPs.

Authors - Machado-Pinilla Rosario R