Recombinant Rabbit CD8B protein (His Tag)

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• Recombinant Rabbit CD8B protein (His Tag) (Eg3262)

Species

Rabbit

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg3262

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Validation Data Gallery

• 

  Purity of Recombinant Rabbit CD8B was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Rabbit CD8B protein Ser19-Cys164 (Accession# G1SX39) with a His tag at the C-terminus.
• GeneID: 100355767
• Accession: G1SX39
• PredictedSize: 17.2 kDa
• SDS-PAGE: 25-32 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD8 is a transmembrane glycoprotein predominantly expressed on cytotoxic T cells and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8b (T-cell surface glycoprotein CD8 beta chain) is an integral membrane glycoprotein that forms disulfide-linked heterodimers with CD8a (CD8 alpha chain). The CD8 alpha/beta heterodimer is the predominant CD8 complex expressed on the cell surface .

References:

1. Hennecke S. et al. (1993). J Biol Chem. 268(35):26607-12.  
2. Xie Q, et al. (2021). Acta Pharm Sin B. 11(6):1365-1378.
3. Wheeler CJ. et al. (1992). Nature. 357(6375):247-9.
4. Terry LA. et al. (1990). Tissue Antigens. 35(2):82-91.