CoraLite®555-conjugated Rabbit IgG control Polyclonal antibody

Rabbit IgG control Polyclonal Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Applications

FC

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-30000

Synonyms

IgG, IgG control, Isotype control, Rabbit IgG, Rabbit IgG isotype, Rabbit Immunoglobulin



Tested Applications

Positive FC detected inCalyculin A treated PC-3 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-30000 targets Rabbit IgG control in FC applications and shows reactivity with samples.

Tested Reactivity
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen N/A
Full Name Rabbit IgG control
Gene Symbol
Gene ID (NCBI)
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570nm
Form Liquid
Purification MethodN/A
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.