Rabenosyn 5 Polyclonal antibody, PBS Only

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• Rabenosyn 5 Polyclonal antibody, PBS Only (22218-1-PBS)

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  WB analysis using 22218-1-AP (same clone as 22218-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22218-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 22218-1-AP (same clone as 22218-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22218-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 22218-1-AP (same clone as 22218-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22218-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 22218-1-AP (same clone as 22218-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22218-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 22218-1-AP (same clone as 22218-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22218-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 22218-1-AP (same clone as 22218-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22218-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 22218-1-AP (same clone as 22218-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22218-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 22218-1-AP (same clone as 22218-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22218-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse heart using 22218-1-AP (same clone as 22218-1-PBS)

  IP result of anti-Rabenosyn 5 (IP:22218-1-AP, 4ug; Detection:22218-1-AP 1:10000) with mouse heart tissue lysate 1920 ug. This data was developed using the same antibody clone with 22218-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 22218-1-AP (same clone as 22218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22218-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 22218-1-AP (same clone as 22218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22218-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 22218-1-AP (same clone as 22218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22218-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 22218-1-AP (same clone as 22218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22218-1-PBS in a different storage buffer formulation.

Rabenosyn 5 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 22218-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

ZFYVE20, 110 kDa protein, FYVE finger-containing Rab5 effector protein rabenosyn-5, Rabenosyn-5, RBSN

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Product Information

22218-1-PBS targets Rabenosyn 5 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17554

  Product name: Recombinant human ZFYVE20 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 437-784 aa of BC106940

  Sequence: LRKAEGWLPLSGGQGQSEDSDPLLQQIHNITSFIRQAKAAGRMDEVRTLQENLRQLQDEYDQQQTEKAIELSRRQAEEEDLQREQLQMLRERELEREREQFRVASLHTRTRSLDFREIGPFQLEPSREPRTHLAYALDLGSSPVPSSTAPKTPSLSSTQPTRVWSGPPAVGQERLPQSSMPQQHEGPSLNPFDEEDLSSPMEEATTGPPAAGVSLDPSARILKEYNPFEEEDEEEEAVAGNPFIQPDSPAPNPFSEEDEHPQQRLSSPLVPGNPFEEPTCINPFEMDSDSGPEAEEPIEEELLLQQIDNIKAYIFDAKQCGRLDEVEVLTENLRELKHTLAKQKGGTD

• Full Name: zinc finger, FYVE domain containing 20
• Calculated Molecular Weight: 784 aa, 89 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC106940
• Gene Symbol: Rabenosyn 5
• Gene ID (NCBI): 64145
• RRID: AB_11182179
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H1K0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.