Recombinant Rat CD80 protein (rFc Tag) (HPLC verified)

Species

Rat

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg3841

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Validation Data Gallery

• 

  Purity of Recombinant Rat CD80 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Rat CD80 was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Rat CD80 protein Ile39-Gln248 (Accession# Q62680) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 25408
• Accession: Q62680
• PredictedSize: 50.2 kDa
• SDS-PAGE: 60-75 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD80, also known as B7, B7.1, or BB1, is a member of the immunoglobulin superfamily with a size of 44-54 kDa and is expressed by a variety of cells, including activated B cells, T cells, macrophages, dendritic cells, and tumor cells. CD80 plays a crucial role in T cell activation.

References:

1. Hathcock KS, et al. J Exp Med. (1994) 180:631–40.
2. Freeman GJ, et al. J Immunol. (1989) 143:2714–22.
3. Li L, et al. Front Immunol. 2024 Nov 7;15:1496992.