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Recombinant Rat CD86 protein (rFc Tag) (HPLC verified)

Species

Rat

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg3586

Validation Data Gallery

  • Purity of Recombinant Rat CD86 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Rat CD86 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Rat CD86 was greater than 90% as determined by SEC-HPLC.

    The purity of Rat CD86 was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Rat CD86 protein Pro30-Thr248 (Accession# O35531) with a rabbit IgG Fc tag at the C-terminus.
GeneID 56822
Accession O35531
PredictedSize 51.3 kDa
SDS-PAGE 55-75 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD86 is a critical costimulatory molecule in antigen-presenting cells including B cells. Induction of CD86 by B cell receptor (BCR) signaling is an important feature of the BCR-induced activation program, although CD86 can also be induced in B cells by CD40 and cytokine signaling.

References:

1. Cyster J. G, et al. (2019). Cell. 177(3):524-540.
2. Hinz M, et al. (2001). Blood. 97(9):2798-807.
3. Attridge K, et al. (2014). J. Immunol. 192(5):2195-201.