Recombinant Rat CD126/IL-6R alpha protein (rFc Tag)

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• Recombinant Rat CD126/IL-6R alpha protein (rFc Tag) (Eg3349)

Species

Rat

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3349

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Validation Data Gallery

• 

  Purity of Recombinant Rat IL-6R alpha was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Rat IL-6R alpha protein Leu20-Pro364 (Accession# NP_058716.2) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 24499
• Accession: NP_058716.2
• PredictedSize: 64.3 kDa
• SDS-PAGE: 70-95 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by various cells during infection, trauma, and immunological challenges. IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6 receptor α (IL-6Rα) subunit (CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6Rα associates with gp130, thus activating the receptor. Expression of gp130 is found in almost all organs while IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells). IL-6Rα is produced by proteolytic shedding and alternative splicing to produce soluble IL-6Rα.

References:

1. Tilg H.et al. (1994). Blood. 1;83(1):113-8. 
2. Heinrich PC.et al. (1998). Biochem J. 1;334 ( Pt 2)(Pt 2):297-314. 
3. Jones SA.et al. (2001). FASEB J. 15(1):43-58. 
4. Horiuchi S.et al. (1994).  Eur J Immunol. 24(8):1945-8.
5. Müllberg J.et al. (1993).  Eur J Immunol. 23(2):473-80. 
6. Rose-John S.et al. (2012). Int J Biol Sci. 8(9):1237-47.