Recombinant Rat CD63 protein (rFc Tag) (HPLC verified)

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• Recombinant Rat CD63 protein (rFc Tag) (HPLC verified) (Eg3216)

Species

Rat

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg3216

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Validation Data Gallery

• 

  Purity of Recombinant Rat CD63 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Rat CD63 was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Rat CD63 protein Arg108-Val203 (Accession# P28648) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 29186
• Accession: P28648
• PredictedSize: 37.2 kDa
• SDS-PAGE: 40-50 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Cluster of differentiation antigen 63 (CD63) is a member of transmembrane-4-superfamily of tetraspanin proteins and is a highly N-glycosylated type III lysosomal membrane protein. CD63 plays important roles in membrane transport, fusion, and protein kinase signaling. CD63 is a widely recognized extracellular vesicle (EV) marker that contributes to EV secretion from cells. CD63 is ubiquitously expressed and often considered to be a late endosomal, lysosomal, as well as an exosomal marker protein. CD63 is known to regulate malignancy of various types of cancers such as melanoma and breast cancer and serves as a potential marker for cancer detection.

References:

1.Song Z. et al. (2020) Molecules. 25(23):5585. 
2.Dey S. et al. (2023) Adv Biol (Weinh). 7(7):e2300078.
3.Yanatori I. et al. (2021) Blood. 138(16):1490-1503.
4.Koh HM. et al. (2020) In Vivo. 34(5):2209-2215.