Recombinant Rat CD68 protein (rFc Tag) (HPLC verified)

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• Recombinant Rat CD68 protein (rFc Tag) (HPLC verified) (Eg1462)

Species

Rat

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg1462

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Validation Data Gallery

• 

  Purity of Recombinant Rat CD68 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Rat CD68 was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Rat CD68 protein Lys20-Ser295 (Accession# Q4FZY1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 287435
• Accession: Q4FZY1
• PredictedSize: 55.8 kDa
• SDS-PAGE: 70-125 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD68, also known as GP110, LAMP4, or SCARD1, is widely expressed in monocyte cell types, such as macrophages, microglia, and osteoclasts. CD68 belongs to the lysosome-associated membrane protein (LAMP) family and is involved in various cellular processes such as autophagy, lipid transport, and aging. Additionally, CD68 is also a member of the scavenger receptor family and plays a critical role in promoting phagocytosis, which is essential for the clearance of cellular debris, pathogens, and the maintenance of tissue homeostasis. CD68 is also a pan-macrophage marker and is the most commonly used universal marker for the identification and quantification of tumor-associated macrophages (TAMs) .

References:

1. Zhang J, et al. (2022). Sci Rep. 12(1):7844.
2. Wilke S, et al. (2012). BMC Biol. 10:62.
3. Song J, et al. (2023). Pathol Res Pract. 249:154739.