Recombinant Rat CXCL1 protein (His Tag)

Species

Rat

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0948

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Validation Data Gallery

• 

  Purity of Recombinant Rat CXCL1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Rat CXCL1 protein Ala25-Lys96 (Accession# P14095) with a His tag at the N-terminus.
• GeneID: 81503
• Accession: P14095
• PredictedSize: 8.9 kDa
• SDS-PAGE: 10-12 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CXC motif chemokine ligand 1 (CXCL1), a CXC chemokine, is also known as growth-regulated (or-related) oncogene-α (Gro-α) and melanoma growth-stimulatory activity (MGSA).It is one of seven chemokines that activate the CXC motif chemokine receptor 2 (CXCR2) and one of the most studied CXC chemokines. CXCL1 is a chemoattractant cytokine. Its most important property is to cause chemotaxis of immune cells, mainly neutrophils, and to a lesser extent CD14+ monocytes and basophils, but not T cells. It also reduces neutrophil apoptosis and thus increases the accumulation of these cells at sites of inflammatory reactions. CXCL1 also causes the chemotaxis of endothelial cells and thus participates in angiogenesis.

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