Recombinant Rat DKK1 protein (rFc Tag)

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• Recombinant Rat DKK1 protein (rFc Tag) (Eg2606)

Species

Rat

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2606

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Validation Data Gallery

• 

  Purity of Recombinant Rat Dkk1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Rat DKK1 protein Thr32-His270 (Accession# A6I0Z0) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 293897
• Accession: A6I0Z0
• PredictedSize: 52.0 kDa
• SDS-PAGE: 58-70 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Dickkopf-1 (DKK1) acts as a natural inhibitor of Wnt/β-catenin pathway, directly binds to the LRP5/6 receptor and Kremen-1/2, leading to internalization of the receptor complex and dampening of Wnt/β-catenin signaling. Dkk1 is abnormally expressed in tumor cells, and abnormal expression of Dkk1 can inhibit cell proliferation or induce apoptosis through pro-apoptotic factors. In vivo, knocking down of DKK1 in the local site of femoral heads in AONFH rats could result in enhanced bone formation and even restoring the blood supply of the femoral heads.

References:

1. D'Amico L, et al. (2016). J Exp Med. May 2;213(5):827-840.
2. Chen J, et al. (2021). Am J Transl Res. Apr 15;13(4):2320-2330.
3. Zhu G, et al. (2021). Cancer Manag Res. Jan 25;13:659-675.