Recombinant Rat IL-12B protein (rFc Tag)

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• Recombinant Rat IL-12B protein (rFc Tag) (Eg3818)

Species

Rat

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3818

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Validation Data Gallery

• 

  Purity of Recombinant Rat IL-12B was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Rat IL-12B protein Met23-Ser335 (Accession# E9PU71) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 64546
• Accession: E9PU71
• PredictedSize: 62.1 kDa
• SDS-PAGE: 62-72 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Interleukin-12B (IL-12B), also known as the natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, or p40, is a protein that plays a crucial role in the immune system. It is one of the subunits of the IL-12 cytokine, which is a heterodimeric protein composed of two subunits: IL-12A (p35) and IL-12B (p40). IL-12B is shared with IL-23, another cytokine, forming part of its structure. IL-12B is involved in stimulating the production of interferon-gamma (IFN-γ) by T cells and NK cells, which is critical for cell-mediated immune responses against intracellular pathogens.

References:

1.Sun L, et al. (2015) Cytokine. 75(2):249-55.
2.Watford WT, et al. (2003) Cytokine Growth Factor Rev. 14(5):361-8.
3.Ma X, et al. (2015) F1000Res. 4:F1000 Faculty Rev-1465.