Recombinant Rat Resistin protein (rFc Tag)

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• Recombinant Rat Resistin protein (rFc Tag) (Eg3408)

Species

Rat

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3408

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Validation Data Gallery

• 

  Purity of Recombinant Rat Resistin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Rat Resistin protein Pro27-Ser114 (Accession# Q8K4J7) with a rabbit IgG Fc tag at the N-terminus.
• GeneID: 246250
• Accession: Q8K4J7
• PredictedSize: 36.6 kDa
• SDS-PAGE: 35-39 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Resistin (also called ADSF: adipocyte secreted factor; FIZZ3: found in inflammatory zone) is a polypeptide hormone first reported from human and rodent adipocyte. Resistin is a member of the resistin-like molecule (RELM) hormone family, which includes RELMα, RELMβ, and RELMγ. Resistin mRNA expression is higher in male than female rats in WAT and BAT. Resistin has been implicated in the pathogenesis of various diseases, including atherosclerosis.

References:

1. Nogueiras R, et al. (2003). FEBS Lett. Jul 31;548(1-3):21-7. 
2. Tovar S, et al. (2005). Eur J Endocrinol. Sep;153(3):R1-5.
3. Li Y, et al. (2021). Front Immunol. Jun 18;12:699807.