Recombinant Rat KIM-1/HAVCR1 protein (His Tag)

Species

Rat

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0597

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Validation Data Gallery

• 

  Purity of Recombinant Rat KIM-1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Rat KIM-1 protein Ser18-Val238 (Accession# O54947) with a His tag at the C-terminus.
• GeneID: 286934
• Accession: O54947
• PredictedSize: 25.7 kDa
• SDS-PAGE: 50-70 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Kidney injury molecule 1 (KIM-1), also known as Hepatitis A virus cellular receptor 1 (HAVCR1), CD365, or T-cell immunoglobulin and mucin domain 1 (TIM-1), is a class I integral membrane glycoprotein, with an ectodomain containing Ig-like domain and a mucin domain. KIM-1 acts as a membrane receptor for hepatitis A virus (HAV). KIM-1 provides a costimulatory signal for T cell activation and inhibits the development of peripheral tolerance. KIM-1 may be involved in the regulation of asthma and allergic diseases. It has been reported that KIM-1 is shed into urine after acute kidney damage and is a marker of renal tubular injury.

References:

1. G Kaplan, et al. (1996) Comparative Study EMBO J. 15(16):4282-96.
2. D Feigelstock, et al. (1998) J Virol. 72(8):6621-8.
3. Jennifer J McIntire, et al. (2003) Nature. 425(6958):576.
4. Takaharu Ichimura, et al. (2003) Am J Physiol Renal Physiol. 286(3):F552-63.
5. Anjali J de Souza, et al. (2005) Proc Natl Acad Sci U S A. 102(47):17113-8.
6. Sarah E Umetsu, et al. (2005) Nat Immunol. 6(5):447-54.