Recombinant Rat TrkA protein (rFc Tag) (HPLC verified)

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• Recombinant Rat TrkA protein (rFc Tag) (HPLC verified) (Eg3210)

Species

Rat

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg3210

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Validation Data Gallery

• 

  Purity of Recombinant Rat TrkA was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Rat TrkA was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Rat TrkA protein Ala33-Pro418 (Accession# P35739-1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 59109
• Accession: P35739-1
• PredictedSize: 68.5 kDa
• SDS-PAGE: 80-120 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

TrkA belongs to the Trk receptor family, which plays a central role in a very broad range of cellular responses and is implicated in a spectrum of human pathologies, including Alzheimer’s, Parkinson’s diseases, pain, and notably cancer. TrkA in particular was first to be identified as a specific receptor for NGF and is activated upon NGF binding.

References:

1. Zhao S, et al. (2020). Commun Biol. 3(1):706.
2. Kaplan DR, et al. (1991). Nature. 350:158–160.
3. Klein R, et al. (1991). Cell. 65:189–197.