entacmaea quadricolor Red fluorescent protein Monoclonal antibody

Red fluorescent protein Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Recombinant Protein and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

5

CloneNo.

1E3C5

Cat no : 67378-1-Ig

Synonyms

GFP-like chromoprotein, Red fluorescent protein eqFP611, RFP, RFP tag

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Validation Data Gallery

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  WB analysis of Recombinant protein using 67378-1-Ig

  RFP tagged protein were subjected to SDS PAGE followed by western blot with 67378-1-Ig (RFP antibody) at dilution of 1:80000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: ag27669

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:100000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below

Product Information

67378-1-Ig targets Red fluorescent protein in WB, IF, ELISA applications and shows reactivity with Recombinant Protein samples.

• Tested Reactivity: Recombinant Protein
• Cited Reactivity: mouse, yeast
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Red fluorescent protein fusion protein Ag27669
• Full Name: Red fluorescent protein
• Calculated Molecular Weight: 26 kDa
• RRID: AB_2882625
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins. Red fluorescent protein from Entacmaea quadricolor. Abs(max)=559 nm; Exhibits a smaller absorbance peak at 525 nm. It has a strong fluorescence emission spectrum which peaks at 611 nm. This antibody can be used to detect the RFP tagged (Entacmaea quadricolor) protein.

Publications

Species	Application	Title
yeast	WB	bioRxiv; Membrane binding of endocytic myosin-1s is inhibited by a class of ankyrin repeat proteins; Authors - Alaina H Willet
mouse	IF	EMBO Rep; Myddosome clustering in IL-1 receptor signaling regulates the formation of an NF-kB activating signalosome; Authors - Fakun Cao
yeast	WB	Mol Biol Cell; Membrane binding of endocytic myosin-1s is inhibited by a class of ankyrin repeat proteins; Authors - Alaina H Willet
	WB	Mol Plant Pathol; Reticulons 3 and 6 interact with viral movement proteins; Authors - Jens Tilsner
	WB	Autophagy; The Valsa mali effector Vm1G-1794 protects the aggregated MdEF-Tu from autophagic degradation to promote infection in apple; Authors - Runmin Che