eqRFP630/eqRFP611 Monoclonal antibody

eqRFP630/eqRFP611 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

recombinant protein and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

7

CloneNo.

1E3C5

Cat no : 67378-1-Ig

Synonyms

Red fluorescent protein, eqFP611, RFP tag, Red fluorescent protein eqFP611, GFP-like chromoprotein

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Validation Data Gallery

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  WB analysis of Recombinant protein using 67378-1-Ig

  RFP tagged protein were subjected to SDS PAGE followed by western blot with 67378-1-Ig (RFP antibody) at dilution of 1:80000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: ag27669

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:100000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 1 publications below

Product Information

67378-1-Ig targets eqRFP630/eqRFP611 in WB, IF, ELISA applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Cited Reactivity: mouse, yeast
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: eqRFP630/eqRFP611 fusion protein Ag27669
• Full Name: eqRFP630/eqRFP611
• Calculated Molecular Weight: 26 kDa
• RRID: AB_2882625
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Red fluorescent proteins (RFPs) is a collective term referring to a heterogenous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages. The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived from Discosoma sp.. It emits red light with a peak wavelength of 593 nm upon excitation by green light (excitation peak at 558 nm). When fused with other proteins, RFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy. RFP611 and RFP 630 is a basic (constitutively fluorescent) red fluorescent protein derived from Entacmaea quadricolor. This antibody is a mouse (IgG2b) monoclonal antibody raised against RFP (eqFP611), it can also recognize eqFP630. This antibody does not recognize DsRed or mRFP.

Publications

Species	Application	Title
yeast	WB	bioRxiv; Membrane binding of endocytic myosin-1s is inhibited by a class of ankyrin repeat proteins; Authors - Alaina H Willet
mouse	IF	EMBO Rep; Myddosome clustering in IL-1 receptor signaling regulates the formation of an NF-kB activating signalosome; Authors - Fakun Cao
yeast	WB	Mol Biol Cell; Membrane binding of endocytic myosin-1s is inhibited by a class of ankyrin repeat proteins; Authors - Alaina H Willet
mouse	WB	Int J Mol Sci; Nmnat1 Deficiency Causes Mitoribosome Excess in Diabetic Nephropathy Mediated by Transcriptional Repressor HIC1; Authors - Kazuhiro Hasegawa
		PNAS Nexus; Ribosome stalling during c-myc translation presents actionable cancer cell vulnerability; Authors - Tejinder Pal Khaket
	WB	Mol Plant Pathol; Reticulons 3 and 6 interact with viral movement proteins; Authors - Jens Tilsner