eqRFP630/eqRFP611 Monoclonal antibody

eqRFP630/eqRFP611 Monoclonal Antibody for WB, ELISA
Cat No. 67378-1-Ig
Clone No.1E3C5

Host / Isotype

Mouse / IgG2b

Reactivity

recombinant protein and More (3)

Applications

WB, IF, ELISA

Red fluorescent protein, eqFP611, RFP tag, Red fluorescent protein eqFP611, GFP-like chromoprotein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inag27669

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:100000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67378-1-Ig targets eqRFP630/eqRFP611 in WB, IF, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Cited Reactivityhuman, mouse, yeast
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen eqRFP630/eqRFP611 fusion protein Ag27669 Predict reactive species
Full Name eqRFP630/eqRFP611
Calculated Molecular Weight 26 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_2882625
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8ISF8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Red fluorescent proteins (RFPs) is a collective term referring to a heterogenous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages. The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived from Discosoma sp.. It emits red light with a peak wavelength of 593 nm upon excitation by green light (excitation peak at 558 nm). When fused with other proteins, RFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy. RFP611 and RFP 630 is a basic (constitutively fluorescent) red fluorescent protein derived from Entacmaea quadricolor. This antibody is a mouse (IgG2b) monoclonal antibody raised against RFP (eqFP611), it can also recognize eqFP630. This antibody does not recognize DsRed or mRFP.

Protocols

Product Specific Protocols
WB protocol for eqRFP630/eqRFP611 antibody 67378-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
yeastWB

bioRxiv

Membrane binding of endocytic myosin-1s is inhibited by a class of ankyrin repeat proteins

Authors - Alaina H Willet
humanWB

PNAS Nexus

Ribosome stalling during c-myc translation presents actionable cancer cell vulnerability

Authors - Tejinder Pal Khaket
WB

Mol Plant Pathol

Reticulons 3 and 6 interact with viral movement proteins

Authors - Jens Tilsner
WB

Autophagy

The Valsa mali effector Vm1G-1794 protects the aggregated MdEF-Tu from autophagic degradation to promote infection in apple

Authors - Runmin Che
mouseIF

EMBO Rep

Myddosome clustering in IL-1 receptor signaling regulates the formation of an NF-kB activating signalosome

Authors - Fakun Cao
yeastWB

Mol Biol Cell

Membrane binding of endocytic myosin-1s is inhibited by a class of ankyrin repeat proteins

Authors - Alaina H Willet
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