Tested Applications
| Positive WB detected in | ag27669 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:100000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67378-1-Ig targets eqRFP630/eqRFP611 in WB, IF, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, mouse, yeast |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27669 Product name: Recombinant entacmaea quadricolor Red fluorescent protein protein Source: e coli.-derived, PET28a Tag: 6*His Sequence: MNSLIKENMRMMVVMEGSVNGYQFKCTGEGDGNPYMGTQTMRIKVVEGGPLPFAFDILATSFMYGSKTFIKHTKGIPDFFKQSFPEGFTWERVTRYEDGGVFTVMQDTSLEDGCLVYHAKVTGVNFPSNGAVMQKKTKGWEPNTEMLYPADGGLRGYSQMALNVDGGGYLSCSFETTYRSKKTVENFKMPGFHFVDHRLERLEESDKEMFVVQHEHAVAKFCDLPSKLGRL Predict reactive species |
| Full Name | eqRFP630/eqRFP611 |
| Calculated Molecular Weight | 26 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2882625 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8ISF8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Red fluorescent proteins (RFPs) is a collective term referring to a heterogenous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages. The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived from Discosoma sp.. It emits red light with a peak wavelength of 593 nm upon excitation by green light (excitation peak at 558 nm). When fused with other proteins, RFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy. RFP611 and RFP 630 is a basic (constitutively fluorescent) red fluorescent protein derived from Entacmaea quadricolor. This antibody is a mouse (IgG2b) monoclonal antibody raised against RFP (eqFP611), it can also recognize eqFP630. This antibody does not recognize DsRed or mRFP.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for eqRFP630/eqRFP611 antibody 67378-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The eqRFP630/eqRFP611 Monoclonal antibody (1E3C5), catalog number 67378-1-Ig, has 9 citations. These appear in journals including Autophagy, Nature Communications, EMBO Reports, International Journal of Molecular Sciences, Molecular Plant Pathology, PNAS Nexus, Molecular Biology of the Cell, and bioRxiv. Research fields span autophagy, STX17 acetylation, lung metastasis, NF-κB signaling, diabetic nephropathy, viral movement proteins, ribosome stalling in cancer, and myosin-1 membrane binding.
| Species | Application | Title |
|---|---|---|
Autophagy The Valsa mali effector Vm1G-1794 protects the aggregated MdEF-Tu from autophagic degradation to promote infection in apple | ||
EMBO Rep Myddosome clustering in IL-1 receptor signaling regulates the formation of an NF-kB activating signalosome | ||
Int J Mol Sci Nmnat1 Deficiency Causes Mitoribosome Excess in Diabetic Nephropathy Mediated by Transcriptional Repressor HIC1 | ||
Reviews
What customers say
Users report mixed results for this RFP antibody in Western Blot. One reviewer found it effective for detecting RFP in mouse tissues and RFP-expressing cells. Another, using a 1:3000 dilution on HEK293T lysates, noted it performed poorly for detecting an mCHERRY-tagged protein, recommending the dedicated mCHERRY antibody (68088-1-Ig) as a better alternative. Overall, the antibody appears reliable for native RFP detection but may have limited cross-reactivity with mCherry variants.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
April (Verified Customer) (08-28-2025) | Product worked well for detecting RFP in mouse tissues with cells containing RFP.
|
Christine (Verified Customer) (08-05-2025) | Tried on total cell lysates of HEK293T transfected with a mCHERRY tagged protein. This RFP antibody was not very good at detecting the mCHERRY tag (mCHERRY antibody from proteintech 68088-1-ig) was better.
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