RyDEN Recombinant monoclonal antibody

• Home
• Products
• Primary Antibodies
• Recombinant Antibodies
• RyDEN Recombinant monoclonal antibody (88428-1-RR)

RyDEN Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 88428-1-RR

Clone No.260958E12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

C19orf66, Interferon-regulated antiviral protein, IRAV, Repressor of yield of DENV protein, SFL

• 

  WB analysis using 88428-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 88428-1-RR (RyDEN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

---

Tested Applications

• Positive WB detected in: THP-1 cells, L02 cells, HuH-7 cells, mouse liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

88428-1-RR targets RyDEN in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag42748

  Product name: Recombinant human C19orf66 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-255 aa of BC035817

  Sequence: MSQEGVELEKSVRRLREKFHGKVSSKKAGALMRKFGSDHTGVGRSIVYGVKQKDGQELSNDLDAQDPPEDMKQDRDIQAVATSLLPLTEANLRMFQRAQDDLIPAVDRQFACSSCDHVWWRRVPQRKEVSRCRKCRKRYEPVPADKMWGLAEFHCPKCRHNFRTHTHSCSAADCYNRREPHVPGTSCAHPKSRKQNHLPKVLHPSNPHISSGSTVATCLSQGGLLEDLDNLILEDLKEEEEEEEEVEDEEGGPRE

• Full Name: chromosome 19 open reading frame 66
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC035817
• Gene Symbol: C19orf66
• Gene ID (NCBI): 55337
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NUL5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The shiftless antiviral inhibitor of ribosomal frameshifting (SHFL) gene was firstly predicted as an ISG in 2011, which is also named as C19orf66, FLJ11286, IRAV, UPF0515, and RyDEN. SHFL showed high expressing level in human liver and spleen tissues, whereas a lower expression is observed in the pancreas and heart. SHFL is a novel ISG that inhibits dengue virus (DENV), West Nile virus (WNV), Zika virus (ZIKV), and Japanese encephalitis virus (JEV) infections. Interestingly, SHFL functions as a broad-spectrum antiviral factor exhibiting suppressive activity against various types of RNA and DNA viruses. (PMID: 36293480, PMID: 26735137, PMID: 38149274)