S1 tag Monoclonal antibody

S1 tag Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

recombinant protein

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A9B5

Cat no : 66165-1-Ig

Synonyms

hepatitis B virus pre S1, NANNPDWDF, S1, S1 tag

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Validation Data Gallery

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  WB analysis of Recombinant protein using 66165-1-Ig

  Recombinant protein were subjected to SDS PAGE followed by western blot with 66165-1-Ig (S1 tag Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66165-1-Ig targets S1 tag in WB, ELISA applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: S1 tag
• RRID: AB_2881561
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S1 tag, with sequence NANNPDWDF, is derived from the hepatitis B virus preS1 region. S1 tag is widely used in various protein expression systems. This antibody can detect the over-expressed fusion proteins containing S1 epitope tag.