S1 tagged protein lysate was subjected to SDS PAGE followed by western blot with CL488-66165 (S1 tag antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
S1 tagged protein lysate was subjected to SDS PAGE followed by western blot with CL488-66165 (S1 tag antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage Conditions
Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
Background Information
S1 tag, with sequence NANNPDWDF, is derived from the hepatitis B virus preS1 region. S1 tag is widely used in various protein expression systems. This antibody can detect the over-expressed fusion proteins containing S1 epitope tag.
Protocols
Product Specific Protocols
WB protocol for CL Plus 488 S1 tag antibody CL488-66165
S1 tagged protein lysate was subjected to SDS PAGE followed by western blot with CL488-66165 (S1 tag antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
S1 tag Monoclonal antibody
Catalog Number
CL488-66165
Citations
-
Dilutions
WB : 1:500-1:1000
Applications
WB
Reactivity
recombinant protein
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
