S100A8 Monoclonal antibody

S100A8 Monoclonal Antibody for IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a


Human and More (2)







Cat no : 66853-1-Ig


60B8AG, CAGA, Calgranulin A, Calprotectin L1L subunit, CFAG, CGLA, CP 10, Cystic fibrosis antigen, L1Ag, MA387, MIF, MRP 8, MRP8, NIF, P8, Protein S100 A8, S100A8, Urinary stone protein band A

Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human cervical cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue

Recommended dilution

Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66853-1-Ig targets S100A8 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen S100A8 fusion protein Ag8517
Full Name S100 calcium binding protein A8
Calculated Molecular Weight 93 aa, 11 kDa
GenBank Accession NumberBC005928
Gene Symbol S100A8
Gene ID (NCBI) 6279
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine.


Product Specific Protocols
IHC protocol for S100A8 antibody 66853-1-IgDownload protocol
IF protocol for S100A8 antibody 66853-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Cell Biol

Giant worm-shaped ESCRT scaffolds surround actin-independent integrin clusters

Authors - Femmy C Stempels

Biochim Biophys Acta Mol Cell Res

IL-17 promotes melanoma through TRAF2 as a scaffold protein recruiting PIAS2 and ELAVL1 to induce EPHA5

Authors - Junfeng Du

Front Immunol

Glomerular Expression of S100A8 in Lupus Nephritis: An Integrated Bioinformatics Analysis.

Authors - Wei Qijiao

J Transl Med

Decoding the colorectal cancer ecosystem emphasizes the cooperative role of cancer cells, TAMs and CAFsin tumor progression

Authors - Rongfang Shen

Transl Oncol

Single-cell RNA sequencing analysis revealed cellular and molecular immune profiles in lung squamous cell carcinoma

Authors - Bo Hao

J Photochem Photobiol B

Hemin protects UVB-induced skin damage through inhibiting keratinocytes apoptosis and reducing neutrophil infiltration

Authors - Fang Bai