S100A8 Monoclonal antibody

S100A8 Monoclonal Antibody for IHC,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1G11C8

Cat no : 66853-1-Ig

Synonyms

60B8AG, CAGA, Calgranulin A, Calprotectin L1L subunit, CFAG, CGLA, CP 10, Cystic fibrosis antigen, L1Ag, MA387, MIF, MRP 8, MRP8, NIF, P8, Protein S100 A8, S100A8, Urinary stone protein band A



Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human cervical cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

66853-1-Ig targets S100A8 in IHC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen S100A8 fusion protein Ag8517
Full Name S100 calcium binding protein A8
Calculated molecular weight 93 aa, 11 kDa
GenBank accession numberBC005928
Gene symbol S100A8
Gene ID (NCBI) 6279
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine.

Protocols

Product Specific Protocols
IHC protocol for S100A8 antibody 66853-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols