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Anti-Human S100A8 Rabbit Recombinant Antibody, PBS Only (98411-3-PBS)

Anti-Human S100A8 Rabbit Recombinant Antibody, PBS Only

Name: Anti-Human S100A8 Rabbit Recombinant Antibody, PBS Only, 1mg
Brand: Proteintech
SKU: 98411-3-PBS-1MG

S100A8 Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. 98411-3-PBS

Clone No.242970C4

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

CAGA, Calgranulin A, Calgranulin-A, Calprotectin L1L subunit, CFAG

Formulation: PBS Only

PBS, Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

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Filter: AllFC (Intra)

FC experiment of human peripheral blood lymphocytes using 98411-3-RR (same clone as 98411-3-PBS)
1x10^6 human peripheral blood lymphocytes were intracellularly stained with 0.25 ug Anti-Human S100A8 Rabbit RecAb (98411-3-RR, Clone: 242970C4),or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 98411-3-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 human peripheral blood lymphocytes were intracellularly stained with 0.25 ug Anti-Human S100A8 Rabbit RecAb (98411-3-RR, Clone: 242970C4),or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 98411-3-PBS in a different storage buffer formulation.
FC experiment of human peripheral blood lymphocytes using 98411-3-RR (same clone as 98411-3-PBS)
1x10^6 human peripheral blood lymphocytes were intracellularly stained with 0.25 ug Anti-Human S100A8 Rabbit RecAb (98411-3-RR, Clone: 242970C4) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 98411-3-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 human peripheral blood lymphocytes were intracellularly stained with 0.25 ug Anti-Human S100A8 Rabbit RecAb (98411-3-RR, Clone: 242970C4) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 98411-3-PBS in a different storage buffer formulation.

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

98411-3-PBS targets S100A8 in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg3752 Product name: Recombinant Human S100A8 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 1-93 aa of BC005928 Sequence: MLTELEKALNSIIDVYHKYSLIKGNFHAVYRDDLKKLLETECPQYIRKKGADVWFKELDINTDGAVNFQEFLILVIKMGVAAHKKSHEESHKE Predict reactive species
Full Name	S100 calcium binding protein A8
Calculated Molecular Weight	93 aa, 11 kDa
GenBank Accession Number	BC005928
Gene Symbol	S100A8
Gene ID (NCBI)	6279
RRID	AB_3746052
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P05109
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine.