Product Information
| Concentration | 0.2 mg/mL |
| Applications | ELISA, WB, Dot blot |
| Host Species | Goat |
| Species Reactivity | Rabbit |
| Physical State | Liquid |
| Suggested Dilution Range | 1:2000-1:10,000 for western blotting with ECL substrates 1:1000-1:20,000 for ELISA and Western blotting with chromogenic substrates |
| Purity | The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
| Storage Buffer | 0.01 M Sodium phosphate, 0.25 M NaCl, 50% glycerol, 3 mg/ml BSA, pH 7.6. |
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Stabilizer | 3 mg/ml BSA |
| Clonality | Polyclonal |
| RRID | AB_2722564 |
| Note | This product is for research use only,not for diagnostic or therapeutic use. |
Documentation
| SDS |
|---|
| SA00001-2-SDS-US |
| SA00001-2-SDS-EU |
Publications
| Title |
|---|
Signal Transduct Target Ther Enhancing the antitumor activity of an engineered TRAIL-coated oncolytic adenovirus for treating acute myeloid leukemia. |
Signal Transduct Target Ther Targeting cancer cell plasticity by HDAC inhibition to reverse EBV-induced dedifferentiation in nasopharyngeal carcinoma. |
Signal Transduct Target Ther Intranasal delivery of replicating mRNA encoding neutralizing antibody against SARS-CoV-2 infection in mice. |
Signal Transduct Target Ther Fusobacterium nucleatum enhances the efficacy of PD-L1 blockade in colorectal cancer. |
Signal Transduct Target Ther A potent human monoclonal antibody with pan-neutralizing activities directly dislocates S trimer of SARS-CoV-2 through binding both up and down forms of RBD. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Gillian (Verified Customer) (03-12-2026) | Great secondary, clean bands with no background when stained at room temperature in 5% milk TBST
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FH Charlotte (Verified Customer) (02-19-2026) | We used it with 2 primary antibodies so far : lamin A/C and Histone H3. Both were nicely revealed. No issue
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FH MALLIKARJUNA (Verified Customer) (11-28-2025) | WORKS VERY GOOD FOR WESTERN BLOT FOR
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FH Delphine (Verified Customer) (10-07-2025) | This secondary antibody performs better than others, allowing clear detection even at a high dilution of 1/6000. It is also reusable after five freeze-thaw cycles.
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FH Rachelle (Verified Customer) (10-02-2025) | Gata3 Primary Antibody is from another vendor. Secondary antibody is HRP-conjugated Goat Anti-Rabbit IgG. This shows 48kDa and 36kDa GATA3 from MCF7 cells. Despite low expression of Gata3 in MCF7, it is detectable with good resolution when HRP (ECL) is used as the readout.
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FH Mei-xin (Verified Customer) (09-29-2025) | This antibody demonstrates excellent specificity, consistently yielding a single clean band without any non-specific signals.
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FH Dhanwini (Verified Customer) (09-24-2025) | GOOD
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FH Robert (Verified Customer) (09-24-2025) | Great for initial probing, if you do stripping and reprobing, expect non-specific bands
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FH Sneha (Verified Customer) (09-24-2025) | GREAT
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FH Harvey (Verified Customer) (09-10-2025) | Western Blot
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FH Samantha (Verified Customer) (08-27-2025) | Excellent and sharp bands, limited non-specific binding
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FH ZIJIAN (Verified Customer) (08-21-2025) | GOOD
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FH Paula (Verified Customer) (02-06-2025) | Secondary ab works 1:1000-1:4000 depending on the primary ab. Overall, good quality.
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FH Macarena (Verified Customer) (10-07-2022) | good results when we are running out of our secondaries of choice
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FH SONIA (Verified Customer) (06-13-2022) |
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FH Brogan (Verified Customer) (02-22-2022) | Used for an ELISA against a custom made polyclonal antibody and worked brilliantly. Will definitely be ordering this secondary antibody again.
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FH Arturo (Verified Customer) (02-17-2022) | Amazing product! I manage to reproduce the same results using the sample now that I was running out of my second antibody for the Western Blots. Now I can take care of the rest of my experiments and finish them for my PhD project.
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FH Paolo (Verified Customer) (03-05-2020) | it works perfectly in ELISA experiments
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FH David (Verified Customer) (06-19-2019) | I use this or an anti-mouse HRP conjugate about everyday, I bought this one after trying the mouse version that was previously here in the lab. I like it very much. Most of the time I get excellent and sharp results. The times I don't I think it's more the experiment than your product.
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