Validation Data Gallery
|Positive WB detected in
|human kidney tissue, A549 cells, mouse kidney tissue, mouse lung tissue, human brain tissue
|Positive IP detected in
|mouse kidney tissue
|Positive IHC detected in
|human brain tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:2000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:20-1:200
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
13033-1-AP targets SACM1L in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|SACM1L fusion protein Ag3682
|SAC1 suppressor of actin mutations 1-like (yeast)
|Calculated molecular weight
|587 aa, 67 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
SACM1L(Suppressor of actin mutations 1-like protein) is also named KIAA0851. It is a phosphoinositide phosphatase that hydrolyzes PtdIns3P and PtdIns4P and has low activity towards PtdIns(3,5)P2.
|Product Specific Protocols
|WB protocol for SACM1L antibody 13033-1-AP
|IHC protocol for SACM1L antibody 13033-1-AP
|IF protocol for SACM1L antibody 13033-1-AP
|IP protocol for SACM1L antibody 13033-1-AP
|Click here to view our Standard Protocols
Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity
Cell Host Microbe
Global mapping of Salmonella enterica-host protein-protein interactions during infection.
An ER Assembly Line of AMPA-Receptors Controls Excitatory Neurotransmission and Its Plasticity.
PtdIns4P restriction by hydrolase SAC1 decides specific fusion of autophagosomes with lysosomes.
Proc Natl Acad Sci U S A
Addiction to Golgi-resident PI4P synthesis in chromosome 1q21.3-amplified lung adenocarcinoma cells.
J Cell Biol
Sensing of nutrients by CPT1C controls SAC1 activity to regulate AMPA receptor trafficking.