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  • KD/KO Validated

SACM1L Polyclonal antibody

SACM1L Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13033-1-AP

Synonyms

KIAA0851, SAC1, SACM1L



Tested Applications

Positive WB detected inhuman kidney tissue, A549 cells, mouse kidney tissue, mouse lung tissue, human brain tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman brain tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

13033-1-AP targets SACM1L in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SACM1L fusion protein Ag3682
Full Name SAC1 suppressor of actin mutations 1-like (yeast)
Calculated molecular weight 587 aa, 67 kDa
Observed molecular weight 60-67 kDa
GenBank accession numberBC016559
Gene symbol SACM1L
Gene ID (NCBI) 22908
RRIDAB_2301284
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SACM1L(Suppressor of actin mutations 1-like protein) is also named KIAA0851. It is a phosphoinositide phosphatase that hydrolyzes PtdIns3P and PtdIns4P and has low activity towards PtdIns(3,5)P2.

Protocols

Product Specific Protocols
WB protocol for SACM1L antibody 13033-1-APDownload protocol
IHC protocol for SACM1L antibody 13033-1-APDownload protocol
IF protocol for SACM1L antibody 13033-1-APDownload protocol
IP protocol for SACM1L antibody 13033-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Cell Res

Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity

Authors - Ying Gao
IP

Cell Host Microbe

Global mapping of Salmonella enterica-host protein-protein interactions during infection.

Authors - Philipp Walch
mouseWB

Neuron

An ER Assembly Line of AMPA-Receptors Controls Excitatory Neurotransmission and Its Plasticity.

Authors - Jochen Schwenk
humanWB

Autophagy

PtdIns4P restriction by hydrolase SAC1 decides specific fusion of autophagosomes with lysosomes.

Authors - Hongjun Zhang
  • KD Validated
humanWB

Proc Natl Acad Sci U S A

Addiction to Golgi-resident PI4P synthesis in chromosome 1q21.3-amplified lung adenocarcinoma cells.

Authors - Lei Shi
humanWB,IF

J Cell Biol

Sensing of nutrients by CPT1C controls SAC1 activity to regulate AMPA receptor trafficking.

Authors - Maria Casas