SAFB2 Polyclonal antibody

SAFB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 11642-1-AP

Synonyms

SAF-B2, SAF B2, KIAA0138

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Validation Data Gallery

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  WB analysis of MCF-7 using 11642-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat pancreas using 11642-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11642-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11642-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 11642-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 11642-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11642-1-AP (SAFB2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of U2OS using 11642-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SAFB2 antibody (11642-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: MCF-7 cells, BxPC-3 cells, HeLa cells, rat pancreas tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

11642-1-AP targets SAFB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SAFB2 fusion protein Ag2230
• Full Name: scaffold attachment factor B2
• Calculated Molecular Weight: 528aa,57 kDa; 953aa,107 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC025279
• Gene Symbol: SAFB2
• Gene ID (NCBI): 9667
• RRID: AB_2301309
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SAFB2, also named as Scaffold attachment factor B2 or KIAA0138, is a 953 amino acid protein, which contains one SAP domain and one RRM (RNA recognition motif) domain. SAFB2 expressed at high levels in the CNS and at low levels in the liver. It also is expressed in a wide number of breast cancer cell lines. SAFB2 binds to scaffold/matrix attachment region (S/MAR) DNA and can function as an estrogen receptor corepressor and can also inhibit cell proliferation.

Publications

Species	Application	Title
mouse	WB,IF	Proteomics; Establishment of a Proteomic Profile Associated with Gonocyte and Spermatogonial Stem Cell Maturation and Differentiation in Neonatal Mice.; Authors - Bo Zheng
mouse	WB	RNA; SAFB associates with nascent RNAs and can promote gene expression in mouse embryonic stem cells; Authors - Rachel E Cherney

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Julia (Verified Customer) (12-05-2024)

Good antibody, seems to be recognising another protein at ~130kDa likely Safb.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: mESC

SAFB2 Antibody Western Blot validation (1:10000 dilution) in mESC (Cat no:11642-1-AP)