Tested Applications
| Positive WB detected in | MCF-7 cells, BxPC-3 cells, HeLa cells, rat pancreas tissue |
| Positive IHC detected in | human breast cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11642-1-AP targets SAFB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2230 Product name: Recombinant human SAFB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-349 aa of BC025279 Sequence: MAETLPGSGDSGPGTASLGPGVAETGTRRLSELRVIDLRAELKKRNLDTGGNKSVLMERLKKAVKEEGQDPDEIGIELEATSKKSAKRCVKGLKMEEEGTEDNGLEDDSRDGQEDMEASLENLQNMGMMDMSVLDETEVANSSAPDFGEDGTDGLLDSFCDSKEYVAAQLRQLPAQPPEHAVDGEGFKNTLETSSLNFKVTPDIEESLLEPENEKILDILGETCKSEPVKEESSELEQPFAQDTSSVGPDRKLAEEEDLFDSAHPEEGDLDLASESTAHAQSSKADSLLAVVKREPAEQPGDGERTDCEPVGLEPAVEQSSAASELAEASSEELAEAPTEAPSPEARDS Predict reactive species |
| Full Name | scaffold attachment factor B2 |
| Calculated Molecular Weight | 528aa,57 kDa; 953aa,107 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC025279 |
| Gene Symbol | SAFB2 |
| Gene ID (NCBI) | 9667 |
| RRID | AB_2301309 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14151 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SAFB2, also named as Scaffold attachment factor B2 or KIAA0138, is a 953 amino acid protein, which contains one SAP domain and one RRM (RNA recognition motif) domain. SAFB2 expressed at high levels in the CNS and at low levels in the liver. It also is expressed in a wide number of breast cancer cell lines. SAFB2 binds to scaffold/matrix attachment region (S/MAR) DNA and can function as an estrogen receptor corepressor and can also inhibit cell proliferation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SAFB2 antibody 11642-1-AP | Download protocol |
| IHC protocol for SAFB2 antibody 11642-1-AP | Download protocol |
| WB protocol for SAFB2 antibody 11642-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SAFB2 Polyclonal antibody (catalog #11642-1-AP) has 4 total citations. These appear in Nucleic Acids Research, Frontiers in Pharmacology, RNA, and Proteomics. The research spans chromatin/RNA biology (X-chromosome inactivation, nascent RNA and gene expression in embryonic stem cells), cancer (SUMOylation biomarkers in pancreatic ductal adenocarcinoma), and reproductive biology (gonocyte and spermatogonial stem cell maturation). Applications include Western blot and immunofluorescence, tested in mouse and human samples.
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res AURKB-driven dissolution of CIZ1-RNA assemblies from the inactive X chromosome in mitosis. | ||
Front Pharmacol SUMOylation substrate encoding genes as prognostic biomarkers in pancreatic ductal adenocarcinoma with functional assessment of SAF-B2 | ||
RNA SAFB associates with nascent RNAs and can promote gene expression in mouse embryonic stem cells | ||
Proteomics Establishment of a Proteomic Profile Associated with Gonocyte and Spermatogonial Stem Cell Maturation and Differentiation in Neonatal Mice. |
Reviews
What customers say
All three reviews are 5-star ratings for Western Blot applications. Users report the antibody performs very well and is highly specific, with one noting it works excellently in RKO cells at 1:10,000 dilution and another confirming strong, specific results in U2OS cells at 1:8,000. One reviewer observed an additional band at ~130 kDa in mESC cells, possibly corresponding to a different protein. Overall, customers are satisfied with the antibody's performance and specificity across multiple cell lines.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tsimafei (Verified Customer) (08-30-2025) | Very good and specific antibody
 |
Aleksandr (Verified Customer) (07-29-2025) | Excellent antibody
|
Julia (Verified Customer) (12-05-2024) | Good antibody, seems to be recognising another protein at ~130kDa likely Safb.
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