SAGE1 Recombinant antibody, PBS Only

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  IF Staining of MDA-MB-453 using 82876-1-RR (same clone as 82876-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MDA-MB-453 cells using SAGE1 antibody (82876-1-RR, Clone: 230094C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82876-1-PBS in a different storage buffer formulation.

• 

  FC experiment of U2OS using 82876-1-RR (same clone as 82876-1-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human SAGE1 (82876-1-RR, Clone:230094C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP)(blue) . Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82876-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82876-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82876-1-RR against Human SAGE1 were performed. The affinity constant is 0.546 nM.

SAGE1 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Cat No. 82876-1-PBS

Clone No.230094C11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

230094C11, Cancer/testis antigen 14, CT14, SAGE, sarcoma antigen 1

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Product Information

82876-1-PBS targets SAGE1 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SAGE1 fusion protein Ag34216
• Full Name: sarcoma antigen 1
• Calculated Molecular Weight: 99kd
• GenBank Accession Number: NM_018666.2
• Gene Symbol: SAGE1
• Gene ID (NCBI): 55511
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NXZ1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Sarcoma antigen 1 (SAGE1) is a cancer/testis antigen that is limited to the spermatogonia and pre-meiotic spermatocytes and localized to the nuclei (PMID: 34465596).