Multi-rAb® SAMHD1 Multi-Recombinant antibody

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  WB analysis using RMX00060

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00060 (SAMHD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using RMX00060

  IP result of anti-SAMHD1 (IP:RMX00060, 4ug; Detection:RMX00060 1:6000) with K-562 cells lysate 2440 ug.

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  IP experiment of HeLa using RMX00060

  IP result of anti-SAMHD1 (IP:RMX00060, 4ug; Detection:RMX00060 1:6000) with HeLa cells lysate 1200 ug.

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  IHC staining of human placenta using RMX00060

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using RMX00060 (SAMHD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using RMX00060

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using RMX00060 (SAMHD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using RMX00060

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SAMHD1 antibody (RMX00060) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  FC experiment of K-562 using RMX00060

  1x10^6 K-562 cells were intracellularly stained with 0.25 ug Multi-rAb® SAMHD1 Multi-Recombinant antibody (RMX00060) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

SAMHD1 Multi-Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. RMX00060

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

DCIP, Dendritic cell-derived IFNG-induced protein, Deoxynucleoside triphosphate triphosphohydrolase SAMHD1, dNTPase, EC:3.1.5.-

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells
• Positive IP detected in: K-562 cells, HeLa cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00060 targets SAMHD1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: SAM domain and HD domain 1
• Calculated Molecular Weight: 626 aa, 72 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC036450
• Gene Symbol: SAMHD1
• Gene ID (NCBI): 25939
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: Q9Y3Z3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SAMHD1, highly expressed by monocytes and monocyte-derived dendritic cells and by monocyte-derived macrophages at a lower extent, is reported as an HIV-1 restriction factor that inhibits the early step of the HIV-1 life cycle. Vpx (virus-like particles containing viral protein X) could overcome this block by interacting with SAMHD1, inducing proteasome-dependent degradation of SAMHD1. Mutations in SAMHD1 cause Aicardi-Goutières syndrome, a genetic encephalopathy with presumed immune pathogenesis. Three alternatively spliced transcripts encoding different isoforms have been described.