SAP18 Polyclonal antibody

SAP18 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13841-1-AP

Synonyms

2HOR0202, SAP18, SAP18P

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Validation Data Gallery

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  WB analysis of K-562 using 13841-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13841-1-AP (SAP18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 13841-1-AP

  IP result of anti-SAP18 (IP:13841-1-AP, 4ug; Detection:13841-1-AP 1:500) with K-562 cells lysate 3200ug.

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  IHC staining of human cervical cancer using 13841-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13841-1-AP (SAP18 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 13841-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13841-1-AP (SAP18 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 13841-1-AP

  Immunofluorescent analysis of Hela cells, using SAP18 antibody 13841-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: K-562 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13841-1-AP targets SAP18 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SAP18 fusion protein Ag4795
• Full Name: Sin3A-associated protein, 18kDa
• Calculated Molecular Weight: 153 aa, 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC030836
• Gene Symbol: SAP18
• Gene ID (NCBI): 10284
• RRID: AB_2301353
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SAP18 is part of the SIN3-repressing complex, which enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism

Publications

Species	Application	Title
human	WB	iScience; Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.; Authors - Mio Iwasaki