Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, mouse liver tissue, HepG2 cells, rat kidney tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17258-1-AP targets SARS2 in WB, IHC, IF/ICC, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10913 Product name: Recombinant human SARS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 169-518 aa of BC042912 Sequence: KLPNQTHPDVPVGDESQARVLHMVGDKPVFSFQPRGHLEIGEKLDIIRQKRLSHVSGHRSYYLRGAGALLQHGLVNFTFNKLLRRGFTPMTVPDLLRGAVFEGCGMTPNANPSQIYNIDPARFKDLNLAGTAEVGLAGYFMDHTVAFRDLPVRMVCSSTCYRAETNTGQEPRGLYRVHHFTKVEMFGVTGPGLEQSSQLLEEFLSLQMEILTELGLHFRVLDMPTQELGLPAYRKFDIEAWMPGRGRFGEVTSASNCTDFQSRRLHIMFQTEAGELQFAHTVNATACAVPRLLIALLESNQQKDGSVLVPPALQSYLGTDRITAPTHVPLQYIGPNQPRKPGLPGQPAVS Predict reactive species |
| Full Name | seryl-tRNA synthetase 2, mitochondrial |
| Calculated Molecular Weight | 518 aa, 58 kDa |
| Observed Molecular Weight | 53 kDa |
| GenBank Accession Number | BC042912 |
| Gene Symbol | SARS2 |
| Gene ID (NCBI) | 54938 |
| RRID | AB_2238863 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NP81 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SARS2 antibody 17258-1-AP | Download protocol |
| IHC protocol for SARS2 antibody 17258-1-AP | Download protocol |
| WB protocol for SARS2 antibody 17258-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SARS2 Polyclonal antibody (Cat# 17258-1-AP) has 3 citations published in Oncogene, J Biol Chem, and EMBO Mol Med. Research fields span hepatocellular carcinoma and Wnt signaling via m5C RNA modification, mitochondrial histidyl-tRNA synthetase function in deafness-associated mutations, and comprehensive fibroblast-based diagnostics for mitochondrial disease. Cited applications include WB and RIP in human samples.
| Species | Application | Title |
|---|---|---|
Oncogene NSUN2 regulates Wnt signaling pathway depending on the m5C RNA modification to promote the progression of hepatocellular carcinoma | ||
J Biol Chem Overexpression of mitochondrial histidyl-tRNA synthetase restores mitochondrial dysfunction caused by a deafness-associated tRNAHis mutation. | ||
EMBO Mol Med Comprehensive functional testing in fibroblasts has strong utility to diagnose mitochondrial disease. |
Reviews
What customers say
The single review for 17258-1-AP is a 5-star rating. The user reported using the antibody for Western Blot at a 1:1500 dilution on fibroblasts, noting that it works very well with strong affinity. Overall, the feedback is positive, highlighting reliable performance and high binding strength in the tested application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Vera (Verified Customer) (07-14-2026) | Antibody works very well, very strong affinity,
|
