SARS2 Polyclonal antibody, PBS Only

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  WB analysis using 17258-1-AP (same clone as 17258-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17258-1-AP (SARS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17258-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 17258-1-AP (same clone as 17258-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 17258-1-AP (SARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17258-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 17258-1-AP (same clone as 17258-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17258-1-AP (SARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17258-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 17258-1-AP (same clone as 17258-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17258-1-AP (SARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17258-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 17258-1-AP (same clone as 17258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17258-1-AP (SARS2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17258-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 17258-1-AP (same clone as 17258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17258-1-AP (SARS2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17258-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 17258-1-AP (same clone as 17258-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 17258-1-AP (SARS2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17258-1-PBS in a different storage buffer formulation.

SARS2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 17258-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:6.1.1.11, mtSerRS, SARS, SARSM, Serine  tRNA ligase

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Product Information

17258-1-PBS targets SARS2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10913

  Product name: Recombinant human SARS2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 169-518 aa of BC042912

  Sequence: KLPNQTHPDVPVGDESQARVLHMVGDKPVFSFQPRGHLEIGEKLDIIRQKRLSHVSGHRSYYLRGAGALLQHGLVNFTFNKLLRRGFTPMTVPDLLRGAVFEGCGMTPNANPSQIYNIDPARFKDLNLAGTAEVGLAGYFMDHTVAFRDLPVRMVCSSTCYRAETNTGQEPRGLYRVHHFTKVEMFGVTGPGLEQSSQLLEEFLSLQMEILTELGLHFRVLDMPTQELGLPAYRKFDIEAWMPGRGRFGEVTSASNCTDFQSRRLHIMFQTEAGELQFAHTVNATACAVPRLLIALLESNQQKDGSVLVPPALQSYLGTDRITAPTHVPLQYIGPNQPRKPGLPGQPAVS

• Full Name: seryl-tRNA synthetase 2, mitochondrial
• Calculated Molecular Weight: 518 aa, 58 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC042912
• Gene Symbol: SARS2
• Gene ID (NCBI): 54938
• RRID: AB_2238863
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NP81
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.