SASS6 Polyclonal antibody

SASS6 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 21377-1-AP

Synonyms

HsSAS 6, SAS 6, SAS6, SASS6



Tested Applications

Positive WB detected inHeLa cells, human testis tissue, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21377-1-AP is SASS6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SASS6 fusion protein Ag15917
Full Name spindle assembly 6 homolog (C. elegans)
Calculated molecular weight 657 aa, 74 kDa
Observed molecular weight 70-74 kDa
GenBank accession numberBC101026
Gene symbol SASS6
Gene ID (NCBI) 163786
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SASS6 antibody 21377-1-APDownload protocol
IHC protocol for SASS6 antibody 21377-1-APDownload protocol
IP protocol for SASS6 antibody 21377-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IHC

Oncol Rep

Knockdown of SASS6 reduces growth of MDA‑MB‑231 triple‑negative breast cancer cells through arrest of the cell cycle at the G2/M phase.

Authors - Lili Du
humanWB

Curr Biol

SAS-6 Association with γ-Tubulin Ring Complex Is Required for Centriole Duplication in Human Cells.

Authors - Hindol Gupta