SASS6 Polyclonal antibody

SASS6 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 21377-1-AP

Synonyms

HsSAS 6, SAS 6, SAS6, SASS6



Tested Applications

Positive WB detected inHeLa cells, human testis tissue, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 21377-1-AP is SASS6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SASS6 fusion protein Ag15917
Full Name spindle assembly 6 homolog (C. elegans)
Calculated molecular weight 657 aa, 74 kDa
Observed molecular weight 70-74 kDa
GenBank accession numberBC101026
Gene symbol SASS6
Gene ID (NCBI) 163786
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SASS6 antibody 21377-1-APDownload protocol
IHC protocol for SASS6 antibody 21377-1-APDownload protocol
IP protocol for SASS6 antibody 21377-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Curr Biol

SAS-6 Association with γ-Tubulin Ring Complex Is Required for Centriole Duplication in Human Cells.

Authors - Hindol Gupta