SC4MOL Recombinant antibody

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  WB analysis using 84742-1-RR

  mouse adipose tissue were subjected to SDS PAGE followed by western blot with 84742-1-RR (SC4MOL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84742-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84742-1-RR against Human SC4MOL were performed. The affinity constant is 12.3 nM.

SC4MOL Recombinant Antibody for WB, ELISA

Cat No. 84742-1-RR

Clone No.241988B11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

ERG25, DESP4, 241988B11

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Tested Applications

• Positive WB detected in: mouse adipose tissue, mouse liver tissue, mouse small intestine tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84742-1-RR targets SC4MOL in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SC4MOL fusion protein Ag34758
• Full Name: sterol-C4-methyl oxidase-like
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC107879
• Gene Symbol: SC4MOL
• Gene ID (NCBI): 6307
• RRID: AB_3672127
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q15800
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SC4MOL(sterol C4-methyl oxidase-like), an intermediate enzyme in the cholesterol biosynthetic pathway, was included in the library based on its representation in a high confidence GEO transcriptional profiling dataset as a transcript that rapidly undergoes significant expression change in response to stimulation or inhibition of EGFR. Among the validated hits that increased cell killing by common EGFR antagonists, SC4MOL was consistently one of the most effective modulators. (PMID: 23125191)