SCAMP5 Recombinant antibody

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  WB analysis using 85669-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85669-2-RR (SCAMP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85669-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85669-2-RR against Human SCAMP5 were performed. The affinity constant is 0.258 nM.

SCAMP5 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85669-2-RR

Clone No.243122F5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

hSCAMP5, Secretory carrier-associated membrane protein 5

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Tested Applications

• Positive WB detected in: mouse brain tissue, 37°C incubated mouse brain tissue, rat brain tissue, 37°C incubated rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85669-2-RR targets SCAMP5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SCAMP5 fusion protein Ag31837
• Full Name: secretory carrier membrane protein 5
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC024700
• Gene Symbol: SCAMP5
• Gene ID (NCBI): 192683
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8TAC9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Secretory carrier-associated membrane protein 5 (SCAMP5) belongs to the to the SCAMP family. Secretory carrier membrane proteins (SCAMPs) constitute a group of membrane transport proteins in plants, insects and mammals. The mammalian genome contains five types of SCAMP genes, namely, SCAMP1-SCAMP5. (PMID:36217917, PMID:19234194). SCAMPs participate in the vesicle cycling fusion of vesicles and cell membranes and play roles in regulating exocytosis and endocytosis, activating synaptic function and transmitting nerve signals. Among these proteins, SCAMP5 is highly expressed in the brain and has direct or indirect effects on the function of the central nervous system (PMID:36217917). SCAMP5 regulates membrane transport, controls the exocytosis of synaptic vesicles and is related to secretion carrier and membrane function. In addition, SCAMP5 plays a major role in the normal maintenance of the physiological functions of nerve cells (PMID:36217917, PMID:33663553). For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.