RPS4X Polyclonal antibody

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RPS4X Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14799-1-AP Copied!

Publications (13)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA, PLA

Synonyms

40S ribosomal protein S4, Small ribosomal subunit protein eS4, X isoform

• 

  WB analysis using 14799-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14799-1-AP (RPS4X antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 14799-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14799-1-AP (RPS4X antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 14799-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14799-1-AP (RPS4X antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 14799-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14799-1-AP (RPS4X antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 14799-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14799-1-AP (RPS4X antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse pancreas using 14799-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14799-1-AP (RPS4X antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human liver using 14799-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14799-1-AP (RPS4X antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HepG2 using 14799-1-AP

  IP result of anti-RPS4X (IP:14799-1-AP, 3ug; Detection:14799-1-AP 1:500) with HepG2 cells lysate 3200ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 14799-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14799-1-AP (RPS4X antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 14799-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14799-1-AP (RPS4X antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 14799-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14799-1-AP (RPS4X antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using 14799-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RPS4X antibody (14799-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, human liver tissue, MCF-7 cells, mouse pancreas tissue, mouse liver tissue, rat liver tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human colon tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14799-1-AP targets RPS4X in WB, IHC, IF/ICC, IP, CoIP, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, xenopus, frog
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6513

  Product name: Recombinant human SCAR protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-263 aa of BC000472

  Sequence: MARGPKKHLKRVAAPKHWMLDKLTGVFAPRPSTGPHKLRECLPLIIFLRNRLKYALTGDEVKKICMQRFIKIDGKVRTDITYPAGFMDVISIDKTGENFRLIYDTKGRFAVHRITPEEAKYKLCKVRKIFVGTKGIPHLVTHDARTIRYPDPLIKVNDTIQIDLETGKITDFIKFDTGNLCMVTGGANLGRIGVITNRERHPGSFDVVHVKDANGNSFATRLSNIFVIGKGNKPWISLPRGKGIRLTIAEERDKRLAAKQSSG

• Full Name: ribosomal protein S4, X-linked
• Calculated Molecular Weight: 263 aa, 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC000472
• Gene Symbol: RPS4X
• Gene ID (NCBI): 6191
• RRID: AB_2238567
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P62701
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPS4X, a X-linked gene in the human, encodes a subunit of ribosomal protein S4, a component of the 40S subunit and 60S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene, RPS4Y2 and the ribosomal protein S4, X-linked (RPS4X). [PMID:8358435]

Publications

What published studies show

RPS4X Polyclonal antibody (cat# 14799-1-AP) has 13 citations published in journals including Cell, Nature Communications, Molecular Therapy, Cell Reports, eLife, Phytomedicine, Biomolecules, BMC Cancer, Aging, Biomarkers in Medicine, American Journal of Medical Genetics C, and Heliyon. Research fields span ribosome biology and translation, cancer (ovarian, urothelial, osteosarcoma, colorectal), neurodevelopment, X-chromosome inactivation and Turner syndrome, gene editing, and protein homeostasis.

Automatically generated from the 13 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell; Regnase-1 and Roquin Regulate a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms.; Authors - Takashi Mino
mouse	WB	Nat Commun; Excess ribosomal protein production unbalances translation in a model of Fragile X Syndrome.; Authors - Sang S Seo
human	WB,CoIP	Mol Ther; Hitchhiking of Cas9 with nucleus-localized proteins impairs its controllability and leads to efficient genome editing of NLS-free Cas9; Authors - Wenfeng Zhang
mouse,human	WB,IHC	Phytomedicine; Ailanthone targets the KMT2A-MEN1 complex to suppress lung metastasis of osteosarcoma; Authors - Jinrong Liang
frog	WB,IF	Cell Rep; On-Site Ribosome Remodeling by Locally Synthesized Ribosomal Proteins in Axons.; Authors - Christine E. Holt
mouse	WB	Cell Rep; Early developmental deletion of forebrain Ank2 causes seizure-related phenotypes by reshaping the synaptic proteome; Authors - Sehyoun Yoon