RPS4X Polyclonal antibody

• Featured Product
• KD/KO Validated

RPS4X Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA, PLA

Conjugate

Unconjugated

Publications

12

Cat no : 14799-1-AP

Synonyms

Small ribosomal subunit protein eS4, X isoform, 40S ribosomal protein S4

---

Validation Data Gallery

• 

  WB analysis of HeLa using 14799-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14799-1-AP (RPS4X antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14799-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14799-1-AP (RPS4X antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14799-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14799-1-AP (RPS4X antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 14799-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14799-1-AP (RPS4X antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 14799-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14799-1-AP (RPS4X antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 14799-1-AP

  IP result of anti-RPS4X (IP:14799-1-AP, 3ug; Detection:14799-1-AP 1:500) with HepG2 cells lysate 3200ug.

• 

  IHC staining of human pancreas cancer using 14799-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14799-1-AP (RPS4X antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 14799-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14799-1-AP (RPS4X antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human colon using 14799-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14799-1-AP (RPS4X antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 14799-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RPS4X antibody (14799-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

---

Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, human liver tissue, MCF-7 cells, mouse pancreas tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

14799-1-AP targets RPS4X in WB, IHC, IF/ICC, IP, CoIP, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, xenopus, frog
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPS4X fusion protein Ag6513
• Full Name: ribosomal protein S4, X-linked
• Calculated Molecular Weight: 263 aa, 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC000472
• Gene Symbol: RPS4X
• Gene ID (NCBI): 6191
• RRID: AB_2238567
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPS4X, a X-linked gene in the human, encodes a subunit of ribosomal protein S4, a component of the 40S subunit and 60S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene, RPS4Y2 and the ribosomal protein S4, X-linked (RPS4X). [PMID:8358435]

Publications

Species	Application	Title
mouse	WB	Cell; Regnase-1 and Roquin Regulate a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms.; Authors - Takashi Mino
mouse	WB	Nat Commun; Excess ribosomal protein production unbalances translation in a model of Fragile X Syndrome.; Authors - Sang S Seo
Xenopus	WB, PLA	Elife; Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.; Authors - Max Koppers
human	IHC	Aging (Albany NY); Construction and external validation of a 5-gene random forest model to diagnose non-obstructive azoospermia based on the single-cell RNA sequencing of testicular tissue; Authors - Ranran Zhou
human	WB,IHC	BMC Cancer; Low expression of the X-linked ribosomal protein S4 in human serous epithelial ovarian cancer is associated with a poor prognosis.; Authors - Tsofack Serges P SP ; KD Validated
		Biomark Med; Low level of the X-linked ribosomal protein S4 in human urothelial carcinomas is associated with a poor prognosis.; Authors - Éric R Paquet