SCCPDH Polyclonal antibody

SCCPDH Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 24941-1-AP

Synonyms

CGI 49, NET11, RP11 439E19.2, SCCPDH

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Validation Data Gallery

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  WB analysis of COLO 320 using 24941-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 24941-1-AP (SCCPDH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 24941-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24941-1-AP (SCCPDH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 24941-1-AP

  IP result of anti-SCCPDH (IP:24941-1-AP, 3ug; Detection:24941-1-AP 1:300) with COLO 320 cells lysate 1700ug.

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  IF Staining of HepG2 using 24941-1-AP

  Immunofluorescent analysis of HepG2 cells using 24941-1-AP (SCCPDH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: COLO 320 cells, HepG2 cells
• Positive IP detected in: COLO 320 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24941-1-AP is SCCPDH Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SCCPDH fusion protein Ag11551
• Full Name: saccharopine dehydrogenase (putative)
• Calculated Molecular Weight: 429 aa, 47 kDa
• Observed Molecular Weight: 40-47 kDa
• GenBank Accession Number: BC026185
• Gene Symbol: SCCPDH
• Gene ID (NCBI): 51097
• RRID: AB_2879811
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.