• Featured Product
  • KD/KO Validated

SCFD1 Polyclonal antibody

SCFD1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12569-1-AP

Synonyms

C14orf163, KIAA0917, RA410, SCFD1, SLY1, SLY1 homolog, Sly1p, STXBP1L2



Tested Applications

Positive WB detected inA549 cells, HEK-293T cells, human kidney tissue, human liver tissue, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12569-1-AP targets SCFD1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, Bacteria
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCFD1 fusion protein Ag3234
Full Name sec1 family domain containing 1
Calculated Molecular Weight 642 aa, 72 kDa
Observed Molecular Weight 66-72 kDa
GenBank Accession NumberBC017734
Gene Symbol SCFD1
Gene ID (NCBI) 23256
RRIDAB_2183266
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCFD1, also named as C14orf163, KIAA0917, STXBP1L2, Sly1p and SLY1, belongs to the STXBP/unc-18/SEC1 family. It is a component of the syntaxin-5 SNARE complex which regulates the early secretory pathway of eukaryotic cells at the level of endoplasmic reticulum (ER) to Golgi transport.(PMID:21242315) SCFD1 plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with COG4. It is involved in vesicular transport between the endoplasmic reticulum and the Golgi.

Protocols

Product Specific Protocols
WB protocol for SCFD1 antibody 12569-1-APDownload protocol
IHC protocol for SCFD1 antibody 12569-1-APDownload protocol
IF protocol for SCFD1 antibody 12569-1-APDownload protocol
IP protocol for SCFD1 antibody 12569-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO J

Direct interaction between the COG complex and the SM protein, Sly1, is required for Golgi SNARE pairing.

Authors - Laufman Orly O
humanWB,IP

Autophagy

Acetylation of SCFD1 regulates SNARE complex formation and autophagosome-lysosome fusion.

Authors - Hong Huang
  • KD Validated
humanWB

J Cell Sci

Autophagic substrate clearance requires activity of the syntaxin-5 SNARE complex.

Authors - Renna Maurizio M
  • KD Validated
BacteriaWB

Microb Pathog

Formononetin alleviates Streptococcus suis infection by targeting suilysin.

Authors - Guizhen Wang
WB

J Appl Microbiol

Silibinin attenuates Streptococcus suis serotype 2 virulence by targeting suilysin.

Authors - Xue Shen