SCIN Polyclonal antibody

• Featured Product
• KD/KO Validated

SCIN Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 11579-1-AP

Synonyms

Scinderin, Adseverin

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Validation Data Gallery

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  WB analysis of human placenta using 11579-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11579-1-AP (SCIN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 11579-1-AP

  IP result of anti-SCIN (IP:11579-1-AP, 4ug; Detection:11579-1-AP 1:300) with human placenta tissue lysate 2800ug.

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  IHC staining of human pancreas cancer using 11579-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11579-1-AP (SCIN antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 11579-1-AP

  Immunofluorescent analysis of HepG2 cells, using SCIN antibody 11579-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: human placenta tissue
• Positive IP detected in: human placenta tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

11579-1-AP targets SCIN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SCIN fusion protein Ag2168
• Full Name: scinderin
• Calculated Molecular Weight: 715 aa, 81 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC021090
• Gene Symbol: SCIN
• Gene ID (NCBI): 85477
• RRID: AB_10641047
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	FEBS Open Bio; Loss of scinderin decreased expression of epidermal growth factor receptor and promoted apoptosis of castration-resistant prostate cancer cells.; Authors - Xiaofeng Lai ; KD Validated
human	WB,IHC	World J Clin Oncol; Scinderin promotes glioma cell migration and invasion via remodeling actin cytoskeleton; Authors - Xin Lin ; KD Validated
human	IF	Cell Biosci; Single-cell RNA sequencing analysis of the temporomandibular joint condyle in 3 and 4-month-old human embryos; Authors - Qianqi Zhu
human	IF	J Am Soc Nephrol; Integrated Single-Cell Transcriptomic Atlas of Human Kidney Endothelial Cells; Authors - Ka Zhang
human	WB	Acta Neuropathol Commun; Synaptic proteomics reveal distinct molecular signatures of cognitive change and C9ORF72 repeat expansion in the human ALS cortex; Authors - Zsofia I. Laszlo