Tested Applications
| Positive WB detected in | human placenta tissue |
| Positive IP detected in | human placenta tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11579-1-AP targets SCIN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2168 Product name: Recombinant human SCIN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-357 aa of BC021090 Sequence: MARELYHEEFARAGKQAGLQVWRIEKLELVPVPQSAHGDFYVGDAYLVLHTAKTSRGFTYRLHFWLGKECSQDESTAAAIFTVQMDDYLGGKPVQNRELQGYESNDFVSYFKGGLKYKAGGVASGLNHVLTNDLTAKRLLHVKGRRVVRATEVPLSWDSFNKGDCFIIDLGTEIYQWCGSSCNKYERLKANQVATGIRYNERKGRSELIVVEEGSEPSELIKVLGEKPELPDGGDDDDIIADISNRKMAKLYMVSDASGSMRVTVVAEENPFSMAMLLSEECFILDHGAAKQIFVWKGKDANPQERKAAMKTAEEFLQQMNYSKNTQIQVLPEGGETPIFKQFFKDWRDKDQSDGFG Predict reactive species |
| Full Name | scinderin |
| Calculated Molecular Weight | 715 aa, 81 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | BC021090 |
| Gene Symbol | SCIN |
| Gene ID (NCBI) | 85477 |
| RRID | AB_10641047 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6U3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SCIN antibody 11579-1-AP | Download protocol |
| IP protocol for SCIN antibody 11579-1-AP | Download protocol |
| WB protocol for SCIN antibody 11579-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SCIN Polyclonal antibody (Cat# 11579-1-AP) has 8 citations published in journals including Advanced Materials, Journal of the American Society of Nephrology, Acta Neuropathologica Communications, Cell Biosciences, European Journal of Pharmacology, American Journal of Surgical Pathology, World Journal of Clinical Oncology, and FEBS Open Biology. Research fields span intestinal microbiome, renal endothelial biology, ALS neurodegeneration, embryonic development, osteoarthritis, renal cell carcinoma, glioma, and prostate cancer.
| Species | Application | Title |
|---|---|---|
Adv Mater Calcium-Powered Probiotics Reconfigure the Intestinal Niche via Biofilm Transformation. | ||
J Am Soc Nephrol Integrated Single-Cell Transcriptomic Atlas of Human Kidney Endothelial Cells | ||
Acta Neuropathol Commun Synaptic proteomics reveal distinct molecular signatures of cognitive change and C9ORF72 repeat expansion in the human ALS cortex | ||
Cell Biosci Single-cell RNA sequencing analysis of the temporomandibular joint condyle in 3 and 4-month-old human embryos | ||
Eur J Pharmacol PDGF-BB inhibits F-actin formation and chondrocyte dedifferentiation in osteoarthritis via oxygen-dependent HIF-1α/SCIN regulation and RhoA/ROCK signaling inhibition. | ||
Am J Surg Pathol Proteomic Analysis Identifies Potential Biomarkers of ELOC-Mutated Renal Cell Carcinoma. |
