SCMH1 Polyclonal antibody

SCMH1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11489-1-AP

Synonyms

Polycomb protein SCMH1, SCMH1, Scml3, Sex comb on midleg homolog 1

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Validation Data Gallery

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  WB analysis using 11489-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11489-1-AP (SCMH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 11489-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 11489-1-AP (SCMH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 11489-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11489-1-AP (SCMH1 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: HT-1080 cells, U-251 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11489-1-AP targets SCMH1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SCMH1 fusion protein Ag1972
• Full Name: sex comb on midleg homolog 1 (Drosophila)
• Calculated Molecular Weight: 599aa,66 kDa; 660aa,73 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC021252
• Gene Symbol: SCMH1
• Gene ID (NCBI): 22955
• RRID: AB_2183702
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.