SCN10A/Nav1.8 Polyclonal antibody

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• SCN10A/Nav1.8 Polyclonal antibody (55334-1-AP)

SCN10A/Nav1.8 Polyclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA

Cat No. 55334-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

SCN10A, hPN3, Nav1.8, PN3, SNS

• 

  IHC staining of mouse brain using 55334-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55334-1-AP (SCN10A/Nav1.8 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 55334-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55334-1-AP (SCN10A/Nav1.8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat Ischiadic nerve using 55334-1-AP

  Immunohistochemical analysis of paraffin-embedded rat Ischiadic nerve tissue slide using 55334-1-AP (SCN10A/Nav1.8 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat Ischiadic nerve using 55334-1-AP

  Immunohistochemical analysis of paraffin-embedded rat Ischiadic nerve tissue slide using 55334-1-AP (SCN10A/Nav1.8 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of SH-SY5Y using 55334-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 55334-1-AP (SCN10A/Nav1.8 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HeLa using 55334-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SCN10A/Nav1.8 (55334-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IHC detected in: mouse brain tissue, rat Ischiadic nerve tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55334-1-AP targets SCN10A/Nav1.8 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: sodium channel, voltage-gated, type X, alpha subunit
• Calculated Molecular Weight: 221 kDa
• GenBank Accession Number: NM_006514
• Gene Symbol: SCN10A
• Gene ID (NCBI): 6336
• RRID: AB_2881309
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y5Y9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sodium channel protein type 10 subunit alpha (SCN10A, also known as Nav1.8) is predominantly expressed in neurons of the dorsal root ganglion (DRG) and trigeminal ganglion (PMID: 8538791; 9169448). Nav1.8 markedly modulates nociceptor excitability, and thereby participates in pain‑processing mechanisms (PMID: 20367448; 11495938). Furthermore, functional SCN10A/Nav1.8 has been detected in intracardiac neurons, revealing a previously unrecognized function of this neuronal sodium channel in the modulation of cardiac electrical activity (PMID: 22723301).

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	J Biomed Sci; Microglial MARCO facilitates Varicella zoster virus uptake and triggers TLR2-mediated neuroinflammation.; Authors - Ji-Soo Lim