SCN10A Polyclonal antibody

SCN10A Polyclonal Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 55334-1-AP

Synonyms

Voltage-gated sodium channel subunit alpha Nav1.8, SNS, PN3, Nav1.8, hPN3

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Validation Data Gallery

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  IF Staining of SH-SY5Y using 55334-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 55334-1-AP (SCN10A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 55334-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SCN10A (55334-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: SH-SY5Y cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55334-1-AP targets SCN10A in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: sodium channel, voltage-gated, type X, alpha subunit
• Calculated Molecular Weight: 221 kDa
• GenBank Accession Number: NM_006514
• Gene Symbol: SCN10A
• Gene ID (NCBI): 6336
• RRID: AB_2881309
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.