SCN4B Polyclonal antibody

SCN4B Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 55438-1-AP

Synonyms

SCN4B, Sodium channel subunit beta 4



Tested Applications

Positive WB detected inmouse heart tissue, mouse brain tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55438-1-AP targets SCN4B in WB, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name sodium channel, voltage-gated, type IV, beta
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberNM_174934
Gene Symbol SCN4B
Gene ID (NCBI) 6330
RRIDAB_2881331
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mammalian sodium channels are heterotrimers, composed of a central, pore-forming alpha subunit and two auxiliary beta subunits (PMID: 11486343). SCN4B (sodium channel subunit beta-4) is one of several sodium channel beta subunits, which modulate channel gating kinetics. SCN4B has an N-terminal signal peptide, a large extracellular domain predicted to form an Ig-like fold, a single transmembrane alpha helix, and a short cytoplasmic C terminus. Defects in SCN4B gene are a cause of long QT syndrome type 10 (LQT10).

Protocols

Product Specific Protocols
WB protocol for SCN4B antibody 55438-1-APDownload protocol
IHC protocol for SCN4B antibody 55438-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols