SCN4B Polyclonal antibody

SCN4B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55438-1-AP

Synonyms

SCN4B, Sodium channel subunit beta 4

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Validation Data Gallery

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  WB analysis of mouse heart using 55438-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55438-1-AP (SCN4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55438-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55438-1-AP (SCN4B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 55438-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55438-1-AP (SCN4B Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55438-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55438-1-AP (SCN4B Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse brain tissue
• Positive IHC detected in: human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55438-1-AP targets SCN4B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: sodium channel, voltage-gated, type IV, beta
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: NM_174934
• Gene Symbol: SCN4B
• Gene ID (NCBI): 6330
• RRID: AB_2881331
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mammalian sodium channels are heterotrimers, composed of a central, pore-forming alpha subunit and two auxiliary beta subunits (PMID: 11486343). SCN4B (sodium channel subunit beta-4) is one of several sodium channel beta subunits, which modulate channel gating kinetics. SCN4B has an N-terminal signal peptide, a large extracellular domain predicted to form an Ig-like fold, a single transmembrane alpha helix, and a short cytoplasmic C terminus. Defects in SCN4B gene are a cause of long QT syndrome type 10 (LQT10).