SCO1 Polyclonal antibody

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• SCO1 Polyclonal antibody (12614-1-AP)

SCO1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 12614-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

SCOD1

• 

  WB analysis using 12614-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human heart using 12614-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of LNCaP using 12614-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human placenta using 12614-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HepG2 using 12614-1-AP

  IP result of anti-SCO1 (IP:12614-1-AP, 4ug; Detection:12614-1-AP 1:1000) with HepG2 cells lysate 1600 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 12614-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12614-1-AP (SCO1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, LNCaP cells, human heart tissue, human placenta tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12614-1-AP targets SCO1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3269

  Product name: Recombinant human SCO1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-301 aa of BC015504

  Sequence: MAMLVLVPGRVMRPLGGQLWRFLPRGLEFWGPAEGTARVLLRQFCARQAEAWRASGRPGYCLGTRPLSTARPPPPWSQKGPGDSTRPSKPGPVSWKSLAITFAIGGALLAGMKHVKKEKAEKLEKERQRHIGKPLLGGPFSLTTHTGERKTDKDYLGQWLLIYFGFTHCPDVCPEELEKMIQVVDEIDSITTLPDLTPLFISIDPERDTKEAIANYVKEFSPKLVGLTGTREEVDQVARAYRVYYSPGPKDEDEDYIVDHTIIMYLIGPDGEFLDYFGQNKRKGEIAASIATHMRPYRKKS

• Full Name: SCO cytochrome oxidase deficient homolog 1 (yeast)
• Calculated Molecular Weight: 301 aa, 34 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC015504
• Gene Symbol: SCO1
• Gene ID (NCBI): 6341
• RRID: AB_2184641
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75880
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human SCO1 and SCO2 are metallochaperones that are essential for the assembly of the catalytic core of cytochrome c oxidase (COX). SCO1 appears to be involved in the folding and assembly of cytochrome oxidase subunit II, and possibly of other proteins of this subcompartment. It is a 33 kDa protein which displays limited homology to members of the DnaJ family(PMID:14731768). It is also named as SCOD1 and belongs to the SCO1/2 family. It can exsit as a homodimer(PMID:16735468). Defects in SCO1 are a cause of mitochondrial complex IV deficiency (MT-C4D).

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Redox Biol; Copper deprivation reprograms antioxidant defense to suppress ferroptosis via SLC7A11.; Authors - Qian Xue
human	WB	Int J Mol Sci; LRPPRC-Driven Oxidative Phosphorylation Is Associated with Elesclomol-Induced Cuproptosis in Ovarian Cancer.; Authors - Ying Wu
mouse,human	WB,IP,IF	Cell Rep; Downregulation of hepatic ceruloplasmin ameliorates NAFLD via SCO1-AMPK-LKB1 complex; Authors - Liping Xie ; KD Validated
human	WB	J Proteome Res; Global Proteomic Analysis Reveals Alterations in Differentially Expressed Proteins between Cardiopathic Lamin A/C Mutations; Authors - Corey L Anderson
		FEBS Lett; Clioquinol induces mitochondrial toxicity in SH-SY5Y neuroblastoma cells by affecting the respiratory chain complex IV and OPA1 dynamin-like GTPase; Authors - Masato Katsuyama