SCO1 Polyclonal antibody

SCO1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12614-1-AP

Synonyms

SCOD1

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Validation Data Gallery

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  WB analysis using 12614-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 12614-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 12614-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12614-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 12614-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12614-1-AP (SCO1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, LNCaP cells, human heart tissue, human placenta tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

12614-1-AP targets SCO1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SCO1 fusion protein Ag3269
• Full Name: SCO cytochrome oxidase deficient homolog 1 (yeast)
• Calculated Molecular Weight: 301 aa, 34 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC015504
• Gene Symbol: SCO1
• Gene ID (NCBI): 6341
• RRID: AB_2184641
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human SCO1 and SCO2 are metallochaperones that are essential for the assembly of the catalytic core of cytochrome c oxidase (COX). SCO1 appears to be involved in the folding and assembly of cytochrome oxidase subunit II, and possibly of other proteins of this subcompartment. It is a 33 kDa protein which displays limited homology to members of the DnaJ family(PMID:14731768). It is also named as SCOD1 and belongs to the SCO1/2 family. It can exsit as a homodimer(PMID:16735468). Defects in SCO1 are a cause of mitochondrial complex IV deficiency (MT-C4D).

Publications

Species	Application	Title
human,mouse	WB,IF,IP	Cell Rep; Downregulation of hepatic ceruloplasmin ameliorates NAFLD via SCO1-AMPK-LKB1 complex; Authors - Liping Xie ; KD Validated
human	WB	J Proteome Res; Global Proteomic Analysis Reveals Alterations in Differentially Expressed Proteins between Cardiopathic Lamin A/C Mutations; Authors - Corey L Anderson