SCO1 Polyclonal antibody, PBS Only

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• SCO1 Polyclonal antibody, PBS Only (12614-1-PBS)

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  WB analysis using 12614-1-AP (same clone as 12614-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12614-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 12614-1-AP (same clone as 12614-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12614-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 12614-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 12614-1-AP (same clone as 12614-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12614-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 12614-1-AP (same clone as 12614-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12614-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 12614-1-AP (same clone as 12614-1-PBS)

  IP result of anti-SCO1 (IP:12614-1-AP, 4ug; Detection:12614-1-AP 1:1000) with HepG2 cells lysate 1600 ug. This data was developed using the same antibody clone with 12614-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 12614-1-AP (same clone as 12614-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12614-1-AP (SCO1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12614-1-PBS in a different storage buffer formulation.

SCO1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 12614-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

SCOD1

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Product Information

12614-1-PBS targets SCO1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3269

  Product name: Recombinant human SCO1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-301 aa of BC015504

  Sequence: MAMLVLVPGRVMRPLGGQLWRFLPRGLEFWGPAEGTARVLLRQFCARQAEAWRASGRPGYCLGTRPLSTARPPPPWSQKGPGDSTRPSKPGPVSWKSLAITFAIGGALLAGMKHVKKEKAEKLEKERQRHIGKPLLGGPFSLTTHTGERKTDKDYLGQWLLIYFGFTHCPDVCPEELEKMIQVVDEIDSITTLPDLTPLFISIDPERDTKEAIANYVKEFSPKLVGLTGTREEVDQVARAYRVYYSPGPKDEDEDYIVDHTIIMYLIGPDGEFLDYFGQNKRKGEIAASIATHMRPYRKKS

• Full Name: SCO cytochrome oxidase deficient homolog 1 (yeast)
• Calculated Molecular Weight: 301 aa, 34 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC015504
• Gene Symbol: SCO1
• Gene ID (NCBI): 6341
• RRID: AB_2184641
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75880
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Human SCO1 and SCO2 are metallochaperones that are essential for the assembly of the catalytic core of cytochrome c oxidase (COX). SCO1 appears to be involved in the folding and assembly of cytochrome oxidase subunit II, and possibly of other proteins of this subcompartment. It is a 33 kDa protein which displays limited homology to members of the DnaJ family(PMID:14731768). It is also named as SCOD1 and belongs to the SCO1/2 family. It can exsit as a homodimer(PMID:16735468). Defects in SCO1 are a cause of mitochondrial complex IV deficiency (MT-C4D).