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SCPEP1 Polyclonal antibody, PBS Only (29781-1-PBS)

SCPEP1 Polyclonal antibody, PBS Only

Name: SCPEP1 Polyclonal antibody, PBS Only,
Brand: Proteintech

SCPEP1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 29781-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

RISC, EC:3.4.16.-, HSCP1, MSTP034, Retinoid-inducible serine carboxypeptidase

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

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Proteintech Guarantee

Filter: AllWBIHC

WB analysis using 29781-1-AP (same clone as 29781-1-PBS)
A431 cells were subjected to SDS PAGE followed by western blot with 29781-1-AP (SCPEP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29781-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse embryo using 29781-1-AP (same clone as 29781-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 29781-1-AP (SCPEP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29781-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse embryo using 29781-1-AP (same clone as 29781-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 29781-1-AP (SCPEP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29781-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

29781-1-PBS targets SCPEP1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag31396 Product name: Recombinant human SCPEP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 221-452 aa of BC072405 Sequence: LLEDKGLAEVSKVAEQVLNAVNKGLYREATELWGKAEMIIEQNTDGVNFYNILTKSTPTSTMESSLEFTQSHLVCLCQRHVRHLQRDALSQLMNGPIRKKLKIIPEDQSWGGQATNVFVNMEEDFMKPVISIVDELLEAGINVTVYNGQLDLIVDTMGQEAWVRKLKWPELPKFSQLKWKALYSDPKSLETSAFVKSYKNLAFYWILKAGHMVPSDQGDMALKMMRLVTQQE Predict reactive species
Full Name	serine carboxypeptidase 1
Calculated Molecular Weight	452 aa, 51 kDa
Observed Molecular Weight	30 kDa, 51 kDa
GenBank Accession Number	BC072405
Gene Symbol	SCPEP1
Gene ID (NCBI)	59342
RRID	AB_2923608
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9HB40
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

SCPEP1 is synthesized as a ~51-55 kDa precursor (zymogen) containing a signal peptide and five N-glycosylation sites. During lysosomal maturation, it undergoes proteolytic cleavage and N-glycan trimming, producing multiple forms that can appear at ~30 kDa on WB. (PMID: 19187242, 16461364)