SCPEP1 Polyclonal antibody, PBS Only

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• SCPEP1 Polyclonal antibody, PBS Only (29781-1-PBS)

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  WB analysis using 29781-1-AP (same clone as 29781-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 29781-1-AP (SCPEP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29781-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse embryo using 29781-1-AP (same clone as 29781-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 29781-1-AP (SCPEP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29781-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse embryo using 29781-1-AP (same clone as 29781-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 29781-1-AP (SCPEP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29781-1-PBS in a different storage buffer formulation.

SCPEP1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 29781-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

29781-1-PBS targets SCPEP1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag31396

  Product name: Recombinant human SCPEP1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 221-452 aa of BC072405

  Sequence: LLEDKGLAEVSKVAEQVLNAVNKGLYREATELWGKAEMIIEQNTDGVNFYNILTKSTPTSTMESSLEFTQSHLVCLCQRHVRHLQRDALSQLMNGPIRKKLKIIPEDQSWGGQATNVFVNMEEDFMKPVISIVDELLEAGINVTVYNGQLDLIVDTMGQEAWVRKLKWPELPKFSQLKWKALYSDPKSLETSAFVKSYKNLAFYWILKAGHMVPSDQGDMALKMMRLVTQQE

• Full Name: serine carboxypeptidase 1
• Calculated Molecular Weight: 452 aa, 51 kDa
• Observed Molecular Weight: 33-51 kDa
• GenBank Accession Number: BC072405
• Gene Symbol: SCPEP1
• Gene ID (NCBI): 59342
• RRID: AB_2923608
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HB40
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SCPEP1 encodes retinoid-inducible serine carboxypeptidase (PMID:12975309). SCPEP1 and Cathepsin A play roles in regulation of vasoconstriction via inactivation of endothelin-1 (PubMed:24586188). The protein is widely expressed during mouse embryogenesis by immunohistochemical (PubMed:16461364). Scpep1 is proteolytically cleaved to a mature enzyme which is smaller than 51 kDa (PMID:16461364).