SCRN1 Polyclonal antibody, PBS Only

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• SCRN1 Polyclonal antibody, PBS Only (14303-1-PBS)

• 

  WB analysis of A549 using 14303-1-AP (same clone as 14303-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 14303-1-AP (SCRN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14303-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 14303-1-AP (same clone as 14303-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14303-1-AP (SCRN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14303-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 14303-1-AP (same clone as 14303-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14303-1-AP (SCRN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14303-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 14303-1-AP (same clone as 14303-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 14303-1-AP (SCRN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14303-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 14303-1-AP (same clone as 14303-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14303-1-AP (SCRN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14303-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 14303-1-AP (same clone as 14303-1-PBS)

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 14303-1-AP (SCRN1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14303-1-PBS in a different storage buffer formulation.

SCRN1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 14303-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

14303-1-PBS targets SCRN1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5413

  Product name: Recombinant human SCRN1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-414 aa of BC040492

  Sequence: MAAAPPSYCFVAFPPRAKDGLVVFGKNSARPRDEVQEVVYFSAADHEPESKVECTYISIDQVPRTYAIMISRPAWLWGAEMGANEHGVCIANEAINTREPAAEIEALLGMDLVRLGLERGETAKEALDVIVSLLEEHGQGGNYFEDANSCHSFQSAYLIVDRDEAWVLETIGKYWAAEKVTEGVRCICSQLSLTTKMDAEHPELRSYAQSQGWWTGEGEFNFSEVFSPVEDHLDCGAGKDSLEKQEESITVQTMMNTLRDKASGVCIDSEFFLTTASGVSVLPQNRSSPCIHYFTGTHDPSRSIFKPFIFVDDVKLVPKTQSPCFGDDDPAKKEPRFQEKPDRRHELYKAHEWARAIIESDQEQGRKLRSTMLELEKQGLEAMEEILTSSEPLDPAEVGDLFYDCVDTEIKFFK

• Full Name: secernin 1
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC040492
• Gene Symbol: SCRN1
• Gene ID (NCBI): 9805
• RRID: AB_2878043
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q12765
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SCRN genes encode protein members of the secernin family including Secernin 1, Secernin 2 and Secernin 3. Function of Secernin 2 and Secernin 3 are so far not well understood. Secernin 1 is a novel 50-kDa cytosolic protein that appears to be involved in the regulation of exocytosis from peritoneal mast cells. Secernin-1 a novel tumor-associated antigen (TAA) and may be a universal marker of different cancer types including gastric cancer, which has been validated by several researches. Measurement of SCRN1 can be used in the early detection of cancer or in the surveillance of patients who undergo surgery.