Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, TT cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16773-1-AP targets SCUBE3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10085 Product name: Recombinant human SCUBE3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 643-992 aa of BC052263 Sequence: YYHGQTEQCVPCPAGTFQEREGQLSCDLCPGSDAHGPLGATNVTTCAGQCPPGQHSVDGFKPCQPCPRGTYQPEAGRTLCFPCGGGLTTKHEGAISFQDCDTKVQCSPGHYYNTSIHRCIRCAMGSYQPDFRQNFCSRCPGNTSTDFDGSTSVAQCKNRQCGGELGEFTGYIESPNYPGNYPAGVECIWNINPPPKRKILIVVPEIFLPSEDECGDVLVMRKNSSPSSITTYETCQTYERPIAFTARSRKLWINFKTSEANSARGFQIPYVTYDEDYEQLVEDIVRDGRLYASENHQEILKDKKLIKAFFEVLAHPQNYFKYTEKHKEMLPKSFIKLLRSKVSSFLRPYK Predict reactive species |
| Full Name | signal peptide, CUB domain, EGF-like 3 |
| Calculated Molecular Weight | 992 aa, 109 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC052263 |
| Gene Symbol | SCUBE3 |
| Gene ID (NCBI) | 222663 |
| RRID | AB_2285430 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IX30 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SCUBE3 is a secreted glycoprotein that belongs to the SCUBE family. Members of the SCUBE family are secreted plasma membrane-associated proteins characterised by a N-terminal signal peptide sequence, multiple EGF domains, a N-linked glycosylated spacer region and a C-terminal CUB region. SCUBE3 may play a role in bone cell biology (PMID: 15234972). It may also has a role in the regulation of cardiac growth and remodeling responses (PMID: 17442284). Besides, SCUBE3 has been reported to be involved in lung cancer progression (PMID: 21441952; 23420440).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SCUBE3 antibody 16773-1-AP | Download protocol |
| WB protocol for SCUBE3 antibody 16773-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SCUBE3 Polyclonal antibody (Cat# 16773-1-AP) has 2 total citations. One appeared in Molecular Cell Endocrinology (2023), studying β-catenin/TCF4-induced SCUBE3 upregulation in ovarian cancer via the HIF-1 signaling pathway using WB. The other was published in Gene (2018), analyzing microRNA and mRNA expression profiles in salivary adenoid cystic carcinoma using IHC. The research fields span ovarian cancer biology and salivary gland tumor molecular profiling.
| Species | Application | Title |
|---|---|---|
Mol Cell Endocrinol β-catenin/TCF4-induced SCUBE3 upregulation promotes ovarian cancer development via HIF-1 signaling pathway
| ||
Gene Comprehensive and in-depth analysis of microRNA and mRNA expression profile in salivary adenoid cystic carcinoma. |
Reviews
What customers say
The single review for this antibody, used in Western Blot, reports that the expected band size is 109 kD, yet the antibody detected a major band at 60 kD. A very weak band around 130 kD was also observed, but it was PNGaseF-insensitive, indicating it is not a glycosylated form of the target protein. Overall, the reviewer expressed dissatisfaction with the specificity and molecular weight recognition of the antibody.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Boyan (Verified Customer) (10-04-2021) | Calculated band is 109 kd. This antibody recognized a major band at 60 kd. There was a very weak band ~130 kd, but was PNGaseF insensitive, suggesting this band is not a glycosylated form of this protein.
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