|Positive WB detected in||HeLa cells, A549 cells, SGC-7901 cells, SH-SY5Y cells, U-251 cells, mouse brain tissue|
|Positive IP detected in||SGC-7901 cells|
|Positive IHC detected in||human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11091-1-AP targets EMAP II in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||EMAP II fusion protein Ag1560|
|Full Name||small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating)|
|Calculated molecular weight||34 kDa|
|Observed molecular weight||34 kDa and 68 kDa|
|GenBank accession number||BC014051|
|Gene ID (NCBI)||9255|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SCYE1(small inducible cytokine subfamily E, member 1) is also named as EMAP2, AIMP1. The human cDNA encodes a 312-amino acid polypeptide that the predicted 34-kDa precursor can be cleaved to produce an active 20-kDa product(PMID:7929199). SCYE1 is secreted as a result of various stimuli, including TNF-α, heat shock, and hypoxia, and that it acts as a multifunctional cytokine on both endothelial and immune cells(PMID:18292511). Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3). It has 2 isoforms produced by alternative splicing and the protein can exsit as a dimer(PMID:11306575).
|Product Specific Protocols|
|WB protocol for EMAP II antibody 11091-1-AP||Download protocol|
|IHC protocol for EMAP II antibody 11091-1-AP||Download protocol|
|IF protocol for EMAP II antibody 11091-1-AP||Download protocol|
|IP protocol for EMAP II antibody 11091-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Int J Mol Med
Changes in the expression of endothelial monocyte‑activating polypeptide II in the rat hippocampus following status epilepticus.
Mass Spectrometric Analysis Identifies AIMP1 and LTA4H as FSCN1-Binding Proteins in Laryngeal Squamous Cell Carcinoma.
J Biol Chem
Structural control of caspase-generated glutamyl-tRNA synthetase by appended noncatalytic WHEP domains.
Trim21-mediated HIF-1α degradation attenuates aerobic glycolysis to inhibit renal cancer tumorigenesis and metastasis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Krishnendu (Verified Customer) (02-27-2020)
Very strong antibody and works nicely for both WB and IP.