SDCCAG1 Polyclonal antibody

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  WB analysis using 11840-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11840-1-AP (SDCCAG1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 11840-1-AP

  IP result of anti-SDCCAG1 (IP:11840-1-AP, 4ug; Detection:11840-1-AP 1:500) with A549 cells lysate 1800ug.

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  IHC staining of human lung cancer using 11840-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11840-1-AP (SDCCAG1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11840-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11840-1-AP (SDCCAG1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

SDCCAG1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 11840-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

NEMF, NY CO 1, SDCCAG1

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells
• Positive IP detected in: A549 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IF: See 2 publications below

Product Information

11840-1-AP targets SDCCAG1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2467

  Product name: Recombinant human SDCCAG1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 48-397 aa of BC020794

  Sequence: EDITLQSGRDELNEELIQEESSEDEGEYEEVRKDQDSVGEMKDEGEETLNYPDTTIDLSHLQPQRSIQKLASKEESSNSSDSKSQSRRHLSAKERREMKKKKLPSDSGDLEALEGKDKEKESTVHIETHQNTSKNVAAVQPMKRGQKSKMKKMKEKYKDQDEEDRELIMKLLGSAGSNKEEKGKKGKKGKTKDEPVKKQPQKPRGGQRVSDNIKKETPFLEVITHELQDFAVDDPHDDKEEQDLDQQGNEENLFDSLTGQPHPEDVLLFAIPICAPYTTMTNYKYKVKLTPGVQKKGKAAKTALNSFMHSKEATAREKDLFRSVKDTDLSRNIPGKVKVSAPNLLNVKRK

• Full Name: serologically defined colon cancer antigen 1
• Calculated Molecular Weight: 1076 aa, 123 kDa
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: BC020794
• Gene Symbol: SDCCAG1
• Gene ID (NCBI): 9147
• RRID: AB_2183413
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60524
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SDCCAG1, also named as NEMF, is a nuclear export mediator. It can be bound to translocon-engaged 60S subunits on native ER membranes. Human SDCCAG1 has been implicated in lung cancers and it may function as a tumor suppressor gene. SDCCAG1 inactivation contributes to the transformed state of human lung cancer cells (PMID:16103875, 10575219). NEMF is also regarded as a potential biomarker for the detection of active and latent TB infections (PMID: 26818387).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nature; UFM1 E3 ligase promotes recycling of 60S ribosomal subunits from the ER; Authors - Paul A DaRosa
	WB	Mol Cell; Convergence of mammalian RQC and C-end rule proteolytic pathways via alanine tailing.; Authors - Anna Thrun ; KO Validated
mouse	WB	Nat Commun; NEMF mutations that impair ribosome-associated quality control are associated with neuromuscular disease.; Authors - Paige B Martin ; KO Validated
human	WB	Cell Rep; NEMF-mediated Listerin-independent mitochondrial translational surveillance by E3 ligase Pirh2 and mitochondrial protease ClpXP; Authors - Liang Lv
human	WB,IF	Acta Neuropathol Commun; Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease; Authors - Suman Rimal
human	WB,IF	Res Sq; Translation stalling induced mitochondrial entrapment of ribosomal quality control related proteins offers cancer cell vulnerability; Authors - Rani Ojha