SDH5 Polyclonal antibody, PBS Only

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• SDH5 Polyclonal antibody, PBS Only (19906-1-PBS)

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  WB analysis using 19906-1-AP (same clone as 19906-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19906-1-AP (SDH5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19906-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 19906-1-AP (same clone as 19906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19906-1-AP (SDH5 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19906-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 19906-1-AP (same clone as 19906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19906-1-AP (SDH5 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19906-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 19906-1-AP (same clone as 19906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 19906-1-AP (SDH5 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19906-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 19906-1-AP (same clone as 19906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 19906-1-AP (SDH5 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19906-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 19906-1-AP (same clone as 19906-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SDH5 antibody (19906-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 19906-1-PBS in a different storage buffer formulation.

SDH5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 19906-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

19906-1-PBS targets SDH5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13893

  Product name: Recombinant human C11orf79 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-166 aa of BC002331

  Sequence: MAVSTVFSTSSLMLALSRHSLLSPLLSVTSFRRFYRGDSPTDSQKDMIEIPLPPWQERTDESIETKRARLLYESRKRGMLENCILLSLFAKEHLQHMTEKQLNLYDRLINEPSNDWDIYYWATEAKPAPEIFENEVMALLRDFAKNKNKEQRLRAPDLEYLFEKPR

• Full Name: chromosome 11 open reading frame 79
• Calculated Molecular Weight: 166 aa, 20 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC002331
• Gene Symbol: SDH5
• Gene ID (NCBI): 54949
• RRID: AB_2878621
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NX18
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SDH5, also named asC11orf79, SDHAF2 and SDHAF2, is a mitochondrial protein needed for the flavination of a succinate dehydrogenase complex subunit required for activity of the complex. SDH5 has been shown to be mutated in patients with paragangliomas, also known as familial non-chromaffin paragangliomas type 2. Catalog#19906-1-AP is a rabbit polyclonal antibody raised against the full-length of human SDH5.