CoraLite®488-conjugated SDHB Monoclonal antibody

SDHB Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

1A2A5

Cat no : CL488-67600

Synonyms

FLJ92337, IP, PGL4, SDH, SDH1, SDHB, SDHIP



Tested Applications

Positive IF detected inhuman liver cancer tissue
For other applications, we recommend the unconjugated version of this antibody, 67600-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-67600 targets SDHB in IF applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SDHB fusion protein Ag29868
Full Name succinate dehydrogenase complex, subunit B, iron sulfur (Ip)
Calculated molecular weight 32 kDa
Observed molecular weight 28-32 kDa
GenBank accession numberBC007840
Gene symbol SDHB
Gene ID (NCBI) 6390
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

SDHB(Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial) is also named as SDH, SDH1 and belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. The SDHD, SDHB, and SDHC genes encode subunits of mitochondrial complex II (succinate dehydrogenase). Mitochondrial complex II is a heterotetrameric complex involved in the aerobic electron transport chain and catalyses the oxidation of succinate to fumarate (Krebs cycle) with transport of electrons to the ubiquinone pool. SDHA and SDHB are the hydrophilic catalytic part of the complex and are highly conserved(J Med Genet 2004;41:e99). Defects in SDHB are a cause of susceptibility to pheochromocytoma (PCC), paragangliomas type 4 (PGL4), paraganglioma and gastric stromal sarcoma (PGGSS) and Cowden-like syndrome (CWDLS).

Protocols

Product Specific Protocols
IF protocol for CL488 SDHB antibody CL488-67600Download protocol
Standard Protocols
Click here to view our Standard Protocols