SEC14L2 Polyclonal antibody

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  WB analysis using 32593-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32593-1-AP (SEC14L2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

SEC14L2 Polyclonal Antibody for WB, ELISA

Cat No. 32593-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Alpha-tocopherol-associated protein, C22orf6, hTAP, KIAA1186, KIAA1658

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Tested Applications

• Positive WB detected in: HEK-293T cells, RKO cells, fetal human brain tissue, mouse brain tissue, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32593-1-AP targets SEC14L2 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SEC14L2 fusion protein Ag35592
• Full Name: SEC14-like 2 (S. cerevisiae)
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC058915
• Gene Symbol: SEC14L2
• Gene ID (NCBI): 23541
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: O76054
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEC14L2 (SEC14-like lipid-binding protein 2) is a cytoplasmic protein that belongs to the lipid-binding protein family. It plays an important role in a variety of cellular processes, including endosome division and lipid metabolism. SEC14L2 is able to bind phosphatidylinositol (PtdIns) and regulate its intracellular distribution, which is essential for normal endosome division. It has been shown that SEC14L2 regulates the endosome division process by promoting the conversion of PtdIns4P to PtdIns3P. In addition, SEC14L2 plays a role in the cholesterol biosynthesis pathway by stimulating squalene monooxygenase activity. In terms of viral infection, SEC14L2 is essential for hepatitis C virus (HCV) replication, and its expression promotes HCV replication in cells.